Detailed information of TA system TA141983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 71219..71904
• Replicon: plasmid unnamed3
• Accession: NZ_CP045495
• Organism: Mesorhizobium sp. NBSH29

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GA830_RS19495
• Protein ID: WP_195165262.1
• Coordinates: 71219..71641 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GA830_RS19500
• Protein ID: WP_195165263.1
• Coordinates: 71638..71904 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GA830_RS19480	67243..68241	+	999	WP_195165137.1	plasmid partitioning protein RepB	-
GA830_RS19485	68412..69755	+	1344	WP_195165260.1	replication initiation protein RepC	-
GA830_RS19490	69942..71111	-	1170	WP_195165261.1	ATP-binding protein	-
GA830_RS19495	71219..71641	-	423	WP_195165262.1	type II toxin-antitoxin system VapC family toxin	Toxin
GA830_RS19500	71638..71904	-	267	WP_195165263.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GA830_RS19505	71996..72160	-	165	Protein_78	integrase	-
GA830_RS19510	72227..75604	-	3378	WP_195165289.1	DEAD/DEAH box helicase	-
GA830_RS19515	75692..76870	-	1179	WP_195165264.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..140849	140849

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-129)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-70)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15841.28 Da
• Isoelectric Point: 4.5120

>T141983 WP_195165262.1 NZ_CP045495:c71641-71219 [Mesorhizobium sp. NBSH29]
VRLLLDTNVLSEVTKPAPEVRVLEWLDSLDEDRSFISVVSIAEIRRGVALMEEGRKREALADWLARDLPMRFEHRVLPVD
EPVAMAWGDLMGLAKRRGRGMSSMDGLIAATAISQDLTLATRNTKDFEDFGIELFDPWMI

Nucleotide

• Length: 423 bp

>T141983 NZ_CP060276:c45368-44988 [Serratia ureilytica]
ATGGTGGCTCAACGGGCGTTATTCGACACCAATATCCTGATCGATTATCTCAACGGCATTCCTCAAGCCAGGGACGTTCT
GGTCGAGTATCACATCAATCCGGCCATCAGCGCCATCACCTGGATGGAAGTGATGGTAGGTGCTAAAAAACAGGGTCCGG
CACTGGAGCTGAAAACACGTCAGTTTTTAGGGCAATTTCTGCTGCTGCCTATTACCGATGAAGTCGCCGAACGCGCCGTA
GAACTGCGCCACTCACAGCATGTGAAACTGCCAGACGCCATTATCTGGGCCACCGCGCAGGTGGGATTCCGGATGCTCAT
CTCCCGCAACCCCAAAGATTTCGGCACTGACAATGGCGTGTTGATGCCTTATCGGCTGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9809.23 Da
• Isoelectric Point: 10.1756

>AT141983 WP_195165263.1 NZ_CP045495:c71904-71638 [Mesorhizobium sp. NBSH29]
MLSSPNQDAKWTVAGAKARLSEVIERAQTSPQTITRNGKPSVVVVSAEEWQRKIARKGTLAEFLLESPLRGTDLVIERLR
DDPRDLPL

Nucleotide

• Length: 267 bp

>AT141983 NZ_CP060276:c45589-45368 [Serratia ureilytica]
ATGACTCGCATCGTGATCGATTTACCTGAAGAAGACCTGCGGCAGCTCGATAATCTGAAAAATATCCGCCACGTTCCCCG
GGCCGAAATCATTCGTCAAGCAGTCTCCCGCTATCTGACGGAGAACCACGTGGAAGATCCCAGCAACGCTTTCGGGCTCT
GGTCCGGGGCGATGGGAGATGGCGTGGAGTACGAAAATCGTCAGCGCGAGGAGTGGGAATAA