Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3213884..3214474 Replicon chromosome
Accession NZ_CP045487
Organism Enterobacter cloacae strain 14240244101

Toxin (Protein)


Gene name pemK Uniprot ID A0A427KSR1
Locus tag GEA60_RS15300 Protein ID WP_047360978.1
Coordinates 3213884..3214216 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A427KRT2
Locus tag GEA60_RS15305 Protein ID WP_020687575.1
Coordinates 3214217..3214474 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GEA60_RS15285 3210043..3211368 + 1326 WP_203461376.1 SidA/IucD/PvdA family monooxygenase -
GEA60_RS15290 3211395..3213584 + 2190 WP_203461377.1 TonB-dependent siderophore receptor -
GEA60_RS15300 3213884..3214216 - 333 WP_047360978.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GEA60_RS15305 3214217..3214474 - 258 WP_020687575.1 antitoxin Antitoxin
GEA60_RS15310 3214888..3215277 - 390 WP_014832566.1 RidA family protein -
GEA60_RS15315 3215419..3216351 + 933 WP_203461378.1 LysR family transcriptional regulator -
GEA60_RS15320 3216447..3216749 - 303 WP_014832568.1 XRE family transcriptional regulator -
GEA60_RS15325 3216733..3217107 - 375 WP_014832569.1 type II toxin-antitoxin system RelE/ParE family toxin -
GEA60_RS15330 3217293..3217499 - 207 Protein_3006 transcriptional regulator -
GEA60_RS15335 3217521..3217748 + 228 Protein_3007 glutathione S-transferase -
GEA60_RS15340 3217798..3218748 - 951 WP_014832572.1 HTH-type transcriptional regulator Cbl -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-85)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11797.68 Da        Isoelectric Point: 10.5834

>T141974 WP_047360978.1 NZ_CP045487:c3214216-3213884 [Enterobacter cloacae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKVTRLPVVVPVTSGGNFARTAGFTVSLDGAGTKTMGIIRCDQPRTI
DMAARNGKRLERVPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T141974 NZ_CP060266:c258973-258638 [Streptococcus pyogenes]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCATCCATTATCACAGCACTAAAGGTTATACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGATCGTTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9437.90 Da        Isoelectric Point: 4.3556

>AT141974 WP_020687575.1 NZ_CP045487:c3214474-3214217 [Enterobacter cloacae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSIDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAT
GKEIL

Download         Length: 258 bp

>AT141974 NZ_CP060266:c259250-258963 [Streptococcus pyogenes]
ATGACTACAGTAAAAAAAAACAGAGCGGTTACCTTTCAAGCTAATAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAGGAAGTGAGGGCTGAACTTGGACTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A427KSR1

Antitoxin

Source ID Structure
AlphaFold DB A0A427KRT2

References