Detailed information of TA system TA141960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA(toxin)
• Location: 3005004..3006787
• Replicon: chromosome
• Accession: NZ_CP045486
• Organism: Enterobacter cloacae strain 99101

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GEA59_RS14310
• Protein ID: WP_203464947.1
• Coordinates: 3005004..3006353 (+)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GEA59_RS14315
• Protein ID: WP_203464948.1
• Coordinates: 3006350..3006787 (+)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEA59_RS14285	3000827..3001762	-	936	WP_203464945.1	tRNA dihydrouridine(16) synthase DusC	-
GEA59_RS14290	3001931..3002326	+	396	WP_063450312.1	CidA/LrgA family protein	-
GEA59_RS14295	3002323..3003018	+	696	WP_203464946.1	CidB/LrgB family autolysis modulator	-
GEA59_RS14300	3003147..3004031	+	885	WP_063868309.1	cytidine deaminase	-
GEA59_RS14305	3004159..3004869	+	711	WP_063450315.1	outer membrane permeability protein SanA	-
GEA59_RS14310	3005004..3006353	+	1350	WP_203464947.1	HipA domain-containing protein	Toxin
GEA59_RS14315	3006350..3006787	+	438	WP_203464948.1	helix-turn-helix domain-containing protein	Antitoxin
GEA59_RS14320	3006824..3007834	-	1011	WP_003859318.1	galactose/methyl galactoside ABC transporter permease MglC	-
GEA59_RS14325	3007850..3009370	-	1521	WP_063450316.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
GEA59_RS14330	3009450..3010448	-	999	WP_063450317.1	galactose/glucose ABC transporter substrate-binding protein MglB	-
GEA59_RS14335	3010744..3011766	-	1023	WP_203464949.1	HTH-type transcriptional regulator GalS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-407)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-129)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 51033.66 Da
• Isoelectric Point: 4.6524

>T141960 WP_203464947.1 NZ_CP045486:3005004-3006353 [Enterobacter cloacae]
METLTVQAYLNETWTDIALIKYPGSEKNDWTTTQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAEASRRYWINALNISELPISQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASAKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHELNAMGFSTIPVDTMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLCALINKITSSHMVQQQGYQFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIRLSPVYDFAPMKADPEGIPRTLKWSLSCESGGEYNFSAIAQALAEWIPPAT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAIGFHYVPNKLSRWGLL

Nucleotide

• Length: 1350 bp

>T141960 NZ_CP060263:714702-715133 [Dickeya zeae]
GTGGCCCAGTGGCAATGTGATTTACGCGTATCCTGGCGTATGCAGTTGTTTTCGCTGCTAACGCACGGATTTCTGATGCT
GATGATCCTGCTGGCTCCCTGGCCGGACGGCTACGCACCGCTGTGGCTCGGGTTAGTGACGCTGGTTGTGTTTGACTTTG
TGCGTAGCCAGCGAAGCATCAAATCGCGCCAGGGGGAAATTTCCCTGCGTGGCGATAACCAACTGCACTGGCAGCAACGT
GACTGGCAGATTGTCCGGCGACCGTGGTTCATGCGCAACGGCGTATTGTTATCGCTACGGGCGGTGGATGGTAAAGGGCA
ACAGCGTTTGTGGCTGGCGTCAGACAGTATGGGGAGTGAGGAATGGCGGCTGTTACGTCAACAGCTGCAGCAGCCTACGC
TGCATGGAACGGAATCTTCCCGCCATCATTAA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16082.47 Da
• Isoelectric Point: 10.7535

>AT141960 WP_203464948.1 NZ_CP045486:3006350-3006787 [Enterobacter cloacae]
MKTPGKTESELLNTLARVRSANKKRQHDEAEDPDTSSVVKRQRVSQGLTRISTLDRQAVLHAAIRDILLGKISQGEALKR
LRVEVLGLKQDEYAKLVNVSRKTLSDVENNRGNYSADVINKIFKPFGLQTGLVPVSKSLIASLFS

Nucleotide

• Length: 438 bp

>AT141960 NZ_CP060263:714455-714721 [Dickeya zeae]
ATGGACATCAATAACAAAGCACGTATTCACTGGGCGTGCAGGCGTGGAATGCGCGAACTGGACATCTCTATCATGCCGTT
TTTCGAGCATGAGTACGATACGCTGAGCGACGACGACAAACAGCAGTTTATTCGTCTGCTGCAGTGTGACGATCCCGACT
TGTTTAATTGGTTGATGAACCACGGCGAGCCGGTTGATCCTGGTTTGAAGCGGATGGTTTCCCTTATTCAGACGCGAAAT
AAAGATCGTGGCCCAGTGGCAATGTGA