Detailed information of TA system TA141951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2349776..2350515
• Replicon: chromosome
• Accession: NZ_CP045486
• Organism: Enterobacter cloacae strain 99101

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: GEA59_RS11265
• Protein ID: WP_193036826.1
• Coordinates: 2349776..2350261 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A4R0FU91
• Locus tag: GEA59_RS11270
• Protein ID: WP_063451299.1
• Coordinates: 2350249..2350515 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEA59_RS11240	2344909..2345667	+	759	WP_203464704.1	DeoR/GlpR family DNA-binding transcription regulator	-
GEA59_RS11245	2345772..2347064	+	1293	WP_203464705.1	glycoside hydrolase	-
GEA59_RS11250	2347150..2347818	+	669	WP_203464706.1	epoxyqueuosine reductase QueH	-
GEA59_RS11255	2348090..2349055	+	966	WP_203464707.1	hypothetical protein	-
GEA59_RS11260	2349071..2349640	+	570	WP_203464708.1	hypothetical protein	-
GEA59_RS11265	2349776..2350261	-	486	WP_193036826.1	GNAT family N-acetyltransferase	Toxin
GEA59_RS11270	2350249..2350515	-	267	WP_063451299.1	DUF1778 domain-containing protein	Antitoxin
GEA59_RS11275	2350609..2351517	-	909	WP_203464709.1	LysR family transcriptional regulator	-
GEA59_RS11280	2351645..2353024	+	1380	WP_203466166.1	DHA2 family efflux MFS transporter permease subunit	-
GEA59_RS11285	2353037..2354032	-	996	WP_203464710.1	DUF2891 domain-containing protein	-
GEA59_RS11290	2354042..2355028	-	987	WP_063866697.1	DUF979 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (34-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17631.35 Da
• Isoelectric Point: 9.9649

>T141951 WP_193036826.1 NZ_CP045486:c2350261-2349776 [Enterobacter cloacae]
VGRVTAPEPLTSVHQLAEFFSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIVLARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFRASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T141951 NZ_CP060244:c1422389-1422135 [Entomobacter blattae]
ATTATGTGGGAAATAAGATTTTCTGAAACAGCTAGTAAAGAATTCAGTAAGCTGGATAAACCAGTGGCCAAAAGGCTTAT
GAACTTTTTGGAAGAACGCGTTGTCACTTTAGAAGACCCTCGTAACCTTGGTAAAGCTCTTCGCGGCCATTTAGGTGAAC
TTTGGTCTTACAGAGTGGGAGACTTTCGTATCATTGCTGATATTCAGGACAAAAAGCTCTGCATCCTTATTCTGAAGACG
GGACACAGAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT141951 WP_063451299.1 NZ_CP045486:c2350515-2350249 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPALEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT141951 NZ_CP060244:c1422616-1422395 [Entomobacter blattae]
ATGACAACCACAGTTCGCCTTAACCAAAATATTGAAGAGCGGCTTGACTTTCTGGCAAAACAGACAGGCCGAACCAAAAC
CTATTATATTGCCCAACTAATTGAAAACGGTTTGGATGAGCTGGAAGATTATTATCTCGCTGCCGATGTTCTGGAAAAAA
TCCGAAAAGGAAAAGAAAAAACCATTTCCTCTTCAGACCTTAAAAAGACTCTTGATCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A4R0FU91