Detailed information of TA system TA141946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4279827..4280431
• Replicon: chromosome
• Accession: NZ_CP045485
• Organism: Enterobacter cloacae strain 88701

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4Q4AAS0
• Locus tag: GEA56_RS20315
• Protein ID: WP_057979982.1
• Coordinates: 4280246..4280431 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GEA56_RS20310
• Protein ID: WP_063449968.1
• Coordinates: 4279827..4280231 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEA56_RS20295	4275739..4277769	+	2031	WP_203463889.1	alpha-amylase	-
GEA56_RS20300	4277900..4279153	+	1254	WP_063449966.1	valine--pyruvate transaminase	-
GEA56_RS20305	4279161..4279616	-	456	WP_203463890.1	4Fe-4S dicluster domain-containing protein	-
GEA56_RS20310	4279827..4280231	-	405	WP_063449968.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GEA56_RS20315	4280246..4280431	-	186	WP_057979982.1	type II toxin-antitoxin system HicA family toxin	Toxin
GEA56_RS20320	4280676..4282214	-	1539	WP_203463891.1	aldehyde dehydrogenase	-
GEA56_RS20325	4282381..4283265	+	885	WP_063865848.1	ROK family protein	-
GEA56_RS20330	4283269..4285107	-	1839	WP_063449971.1	selenocysteine-specific translation elongation factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6852.08 Da
• Isoelectric Point: 11.5191

>T141946 WP_057979982.1 NZ_CP045485:c4280431-4280246 [Enterobacter cloacae]
VKSADIITVLVSHGWKCVRTKGSHHQFRHPVQKGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Nucleotide

• Length: 186 bp

>T141946 NZ_CP060243:5527171-5527353 [Pseudomonas aeruginosa]
ATGAAGTTCAGCGAATTCAGACGATGGTTGAAGGCCCAAGGGGTGACCTTCGAAGCCGGCAAGGGAAGCCACTTCAAGAT
CACCGCCCCGAACGGCAAACAGACCACCTTCGCGGACCACGGAGCTAAGGAAATGCCAGAACCGACCCGCAAGGCGATCA
TCAAGCAACTGGGGCTCAAATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 15049.01 Da
• Isoelectric Point: 4.3984

>AT141946 WP_063449968.1 NZ_CP045485:c4280231-4279827 [Enterobacter cloacae]
MFYPAYIHSDSDGSASGFFPDVPGCFFAGDSLDDAFQDARDALTAHFEALFEMDEMLPLPGMVEAHLASHPEDFIGGQWL
LVDINMKQFEGRVERINITIPRRLLVKIDSFVSEHPQFTNRSAFLAEAARRVLP

Nucleotide

• Length: 405 bp

>AT141946 NZ_CP060243:5527400-5527828 [Pseudomonas aeruginosa]
ATGTACGACTATGCAATCCGTTTCGAACAGGACGATAGCGCTCCTGGCGTCGCCGTTTTCTGCAGAGACCTGCCGGAACT
GAACAGCTACGGCGACGATAAGGCCCATGCAATCAGCGAGGCGGTTGACGCCATCGAGTCAACCCTCTCGCTGTACGTTG
ATCAGCGCCGAGAAATCCCCGCAGCCAGCCAGGCACAACCAGGCGAGCGCGTTATCCATCTGCCGGCGGTTACCGTTGCG
AAGGTCGCGCTCTGGAACGAAATGATCCGTCGAGATATGCGAAAAGCTGACCTCTGCCGGCTCCTCGGGATCGCGCAGAT
CCAGGGTGACAGGCTCGTCGACTTCCTCCACAACACCAAGATGGAAGCCATGGAGAACGCGCTATCCGCCCTCGGCCTCC
GCCTATCAGTGAATATCGAGGCGGCATAG

Structures

Toxin

• AlphaFold DB: A0A4Q4AAS0