Detailed information of TA system TA141925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/HTH_10(toxin)
• Location: 1596181..1597156
• Replicon: chromosome
• Accession: NZ_CP045484
• Organism: Sulfurisphaera ohwakuensis strain TA-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q970K2
• Locus tag: D1869_RS08930
• Protein ID: WP_010979649.1
• Coordinates: 1596181..1596795 (-)
• Length: 205 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: D1869_RS08935
• Protein ID: WP_156014797.1
• Coordinates: 1596809..1597156 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1869_RS08910 (D1869_08945)	1591407..1592471	+	1065	WP_156014793.1	hypothetical protein	-
D1869_RS08915 (D1869_08950)	1592485..1593939	+	1455	WP_156014794.1	4Fe-4S binding protein	-
D1869_RS08920 (D1869_08955)	1594320..1594577	-	258	WP_156014795.1	DUF4898 domain-containing protein	-
D1869_RS08925 (D1869_08960)	1594918..1595955	-	1038	WP_156014796.1	nitric oxide response protein	-
D1869_RS08930 (D1869_08965)	1596181..1596795	-	615	WP_010979649.1	helix-turn-helix domain-containing protein	Toxin
D1869_RS08935 (D1869_08970)	1596809..1597156	-	348	WP_156014797.1	DUF488 domain-containing protein	Antitoxin
D1869_RS08940 (D1869_08975)	1597555..1598001	-	447	WP_156014798.1	cation-binding protein	-
D1869_RS08945 (D1869_08980)	1598197..1599429	+	1233	WP_156014799.1	RNA-guided endonuclease TnpB family protein	-
D1869_RS08950 (D1869_08985)	1599572..1600426	+	855	WP_156014800.1	amidohydrolase family protein	-
D1869_RS08955 (D1869_08990)	1600693..1601823	-	1131	WP_156014801.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterioopsin activator-type, HTH domain (145-197)

Antitoxin

• Protein of unknown function DUF488 (3-108)

Sequences

Toxin

Protein

• Length: 205 a.a.
• Molecular weight: 23087.00 Da
• Isoelectric Point: 9.2970

>T141925 WP_010979649.1 NZ_CP045484:c1596795-1596181 [Sulfurisphaera ohwakuensis]
MPKSPFEVTLLIEDHPCEVMKLISTMGLKASVENVKLGDNVTDHIVLFDNKVKNEDVLKLKSGNSKVLRLSDNRIWVRTN
GCSVCKVLYTSDVVVEKIKVVKERTLLYTLLIPNTSSLKEFLSKLTSQGVKVTVISTNEITGNELTERQMEILKLAYRLG
YFDDDRGITLTELANRLNVSAPTLEEILRRALRKVVKYYLDKVG

Nucleotide

• Length: 615 bp

>T141925 NZ_CP060240:c4684325-4683915 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTTGACACCAACATCTGCATCTTCACTATCAAGAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGACCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATCGCCGGACACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCACCC
CAATCGTATAG

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13705.99 Da
• Isoelectric Point: 9.9648

>AT141925 WP_156014797.1 NZ_CP045484:c1597156-1596809 [Sulfurisphaera ohwakuensis]
MIKIKRIYDPVEKDDGIRILVDRLWPRGVRKDKVDVWLKDIAPSDELRTWFNHDPNKWEEFKKKYFEELSKNPKLDILLQ
LIKKGENVTLLYASKSPYNNAVALKEFLDKIFKAS

Nucleotide

• Length: 348 bp

>AT141925 NZ_CP060240:c4684555-4684325 [Pseudomonas aeruginosa]
GTGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCCGTCGCGCTTCCTGATGACGT
TAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGTTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Structures

Toxin

• AlphaFold DB: A0A832TIR3