Detailed information of TA system TA141908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 1721025..1722133
• Replicon: chromosome
• Accession: NZ_CP045472
• Organism: Staphylococcus aureus strain ZY05

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: GAY69_RS08145
• Protein ID: WP_000233000.1
• Coordinates: 1721264..1722133 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: GAY69_RS08140
• Protein ID: WP_000205227.1
• Coordinates: 1721025..1721249 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAY69_RS14120	1716614..1716784	+	171	WP_000713595.1	hypothetical protein	-
GAY69_RS08115	1716798..1717400	+	603	WP_001062586.1	recombinase family protein	-
GAY69_RS08120	1717416..1718093	+	678	Protein_1590	DNA topoisomerase III	-
GAY69_RS08125	1718605..1718901	+	297	WP_002360685.1	hypothetical protein	-
GAY69_RS08130	1718902..1719318	+	417	WP_000323438.1	recombinase	-
GAY69_RS08135	1719338..1720882	+	1545	WP_002390960.1	recombinase family protein	-
GAY69_RS08140	1721025..1721249	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
GAY69_RS08145	1721264..1722133	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
GAY69_RS08150	1722114..1722848	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
GAY69_RS08155	1722881..1723789	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
GAY69_RS08160	1723786..1724266	+	481	Protein_1598	GNAT family N-acetyltransferase	-
GAY69_RS08165	1724359..1725153	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
GAY69_RS08175	1725695..1725778	+	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
GAY69_RS08180	1725903..1726640	+	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	cat(pC233) / ant(6)-Ia / aph(3')-III / erm(B)	-	1708361..1731556	23195
	-	flank	IS/Tn	ant(6)-Ia / aph(3')-III / erm(B)	-	1722881..1731556	8675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T141908 WP_000233000.1 NZ_CP045472:1721264-1722133 [Staphylococcus aureus]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T141908 NZ_CP060193:2793631-2794377 [Bacillus sp. PAMC26543]
ATGCTTTTATTCTTTCAAATCATGGTCTGGTGCACTATGGCCGCTTTAGGCTTATACGTATACGCCACATGGCGGTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGAAAAACATGGTATTTGCTGTTTGTTTTGGGCGCCATGATATACTGGA
CATATGATCCCTCTTCCCTGTTCACAAACTGGGAACGGTATGTCATTGTCGCTGTCAGCTTTGCTTTAATTGACGCTTTT
ATCTTTTTGAGCGCTTATGTGAAAAAACTGGCCGGCAGTGAACTGGAAACAGACACAAGAGAAATTCTCGAAGAAAACAA
CGAAATGCTCCACATGTATCTCGATCGGCTGAAAACATACCAATATCTATTGAAAAACGAACCGATTCATGTTTATTATG
GAAGTATAGAAGCATATGCTGAAGGAATTGATAAACTGCTGAAAACCTATGCTGATAAAATGAATCTGACGGCCAGCCTT
TGTCACTATTCGACACAGTCTGATAAAGACCGGTTAACCGAGCACATGGAGGATCCCGCTGACGTGCAAACGAGGCTGGA
TCGAAAGGATGTCTATTACGACCAGTATGGAAAAGTAGTGCTCATTCCCTTTACAATTGAGACACAGAGCTATGTCATCA
AACTGACGTCTGACAGCATTGTAACGGAATTTGATTATTTGCTATTCACATCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT141908 WP_000205227.1 NZ_CP045472:1721025-1721249 [Staphylococcus aureus]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT141908 NZ_CP060193:2794377-2794547 [Bacillus sp. PAMC26543]
ATGGAAAACGCGTTTCAAAACGGATGCCATCCGCGGGCAAAGAAACCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCACACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGATGAGTATAAACGAA
AAAAAGCATGA

Structures

Toxin

• AlphaFold DB: R3JIN1