Detailed information of TA system TA141905

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 9127..9659
• Replicon: plasmid pSAW1-3
• Accession: NZ_CP045470
• Organism: Staphylococcus aureus strain SAW1

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: Q93CD4
• Locus tag: GBR75_RS13935
• Protein ID: WP_001103942.1
• Coordinates: 9339..9659 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: -
• Locus tag: GBR75_RS13930
• Protein ID: WP_001058486.1
• Coordinates: 9127..9336 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBR75_RS13885	4157..5377	-	1221	WP_160210569.1	site-specific integrase	-
GBR75_RS13890	5466..6193	-	728	Protein_8	staphylococcal enterotoxin type K	-
GBR75_RS13895	6217..6943	-	727	Protein_9	staphylococcal enterotoxin type Q	-
GBR75_RS13900	7013..7443	-	431	Protein_10	hypothetical protein	-
GBR75_RS13905	7460..7918	-	459	WP_000281657.1	ImmA/IrrE family metallo-endopeptidase	-
GBR75_RS13910	7930..8262	-	333	WP_001260004.1	helix-turn-helix transcriptional regulator	-
GBR75_RS13915	8452..8712	+	261	WP_160210499.1	helix-turn-helix domain-containing protein	-
GBR75_RS13920	8705..8976	+	272	Protein_14	helix-turn-helix domain-containing protein	-
GBR75_RS13925	8988..9134	+	147	WP_000784875.1	hypothetical protein	-
GBR75_RS13930	9127..9336	+	210	WP_001058486.1	hypothetical protein	Antitoxin
GBR75_RS13935	9339..9659	+	321	WP_001103942.1	DUF1474 family protein	Toxin
GBR75_RS13940	9723..10635	+	913	Protein_18	primase alpha helix C-terminal domain-containing protein	-
GBR75_RS13945	10604..12073	+	1470	Protein_19	virulence-associated protein E	-
GBR75_RS13950	12359..12721	+	363	WP_001039168.1	hypothetical protein	-
GBR75_RS13955	12723..13007	+	285	WP_000998181.1	hypothetical protein	-
GBR75_RS13960	13004..13643	+	640	Protein_22	pathogenicity island protein	-
GBR75_RS13965	14162..14503	+	342	WP_001190615.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	seb / selk / selq	1..15908	15908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12694.22 Da
• Isoelectric Point: 4.7419

>T141905 WP_001103942.1 NZ_CP045470:9339-9659 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFSELIQKF
HEIEKASSENFDEESDDAKNSIKVAE

Nucleotide

• Length: 321 bp

>T141905 NZ_CP060191:223277-223627 [Bacillus sp. PAMC28571]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCAGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTGATGCAAAGCGCTACGGCTTTGAGCGAGATTCTGTCATTCTCTTGGAGCAGATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8252.31 Da
• Isoelectric Point: 4.3718

>AT141905 WP_001058486.1 NZ_CP045470:9127-9336 [Staphylococcus aureus]
MNNEQKEVIEHLVYQLELSVMNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT141905 NZ_CP060191:222991-223272 [Bacillus sp. PAMC28571]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTACCGGAGGCTTTGATGTCTGAAATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Structures

Toxin

• AlphaFold DB: Q93CD4