Detailed information of TA system TA141899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2105699..2106213
• Replicon: chromosome
• Accession: NZ_CP045468
• Organism: Staphylococcus aureus strain SAW1

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: GBR75_RS10325
• Protein ID: WP_000581792.1
• Coordinates: 2105699..2105908 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A0D1GBN9
• Locus tag: GBR75_RS10330
• Protein ID: WP_000138934.1
• Coordinates: 2105920..2106213 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBR75_RS10305	2101207..2102565	-	1359	WP_000611468.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
GBR75_RS10310	2102580..2103650	-	1071	WP_000159641.1	D-alanine--D-alanine ligase	-
GBR75_RS10315	2103968..2105170	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
GBR75_RS10320	2105434..2105571	-	138	WP_000828354.1	hypothetical protein	-
GBR75_RS10325	2105699..2105908	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
GBR75_RS10330	2105920..2106213	-	294	WP_000138934.1	copper-sensing transcriptional repressor CsoR	Antitoxin
GBR75_RS10335	2106379..2107863	+	1485	WP_000571549.1	cardiolipin synthase	-
GBR75_RS10340	2107891..2108538	+	648	WP_001187610.1	HD domain-containing protein	-
GBR75_RS10360	2109353..2110225	-	873	WP_000725802.1	membrane protein insertase YidC	-
GBR75_RS10365	2110311..2110952	-	642	WP_000483147.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T141899 WP_000581792.1 NZ_CP045468:c2105908-2105699 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T141899 NZ_CP060160:962503-962853 [Staphylococcus lugdunensis]
ATGATTAGACGCGGCGATGTATATTTAGCTGATTTATCACCGGTACAAGGGTCTGAACAAGGGGGAGTAAGACCAGTTGT
TATCATTCAAAATGACACCGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCAATTACTGGAAGGATTAATAAAGCGA
AAATACCCACTCATGTGGAAATAGGTAAAAAGAAATATAAGTTAGATAAAGATTCTGTTATTCTGTTAGAGCAAATACGA
ACATTAGATAAAAAAAGATTAAAAGAAAAACTTACTTATCTCTCTGAGGATAAAATGAAAGAAGTAGATCATGCCCTAGA
CATTAGTCTAGGTTTACATAACTATCATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11185.77 Da
• Isoelectric Point: 6.2243

>AT141899 WP_000138934.1 NZ_CP045468:c2106213-2105920 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT141899 NZ_CP060160:962336-962506 [Staphylococcus lugdunensis]
ATGCTAACTTTTAATCAAAATCGTAGTCATAGTCTAGAACAGTCATTAAAAGAAGGGTATGCTCAGATGGCAGACCTTAA
TCTCTCCCTAGCAACAGAGGCTTTTCCAATTGAATGTGAAGCTTGTGATTGCAATGAAACTTATCTAACTTCCAATCATC
AGAGTGAATGA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: A0A0D1GBN9