Detailed information of TA system TA141898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2085959..2086488
• Replicon: chromosome
• Accession: NZ_CP045468
• Organism: Staphylococcus aureus strain SAW1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GBR75_RS10235
• Protein ID: WP_000621175.1
• Coordinates: 2085959..2086321 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: GBR75_RS10240
• Protein ID: WP_000948331.1
• Coordinates: 2086318..2086488 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBR75_RS10215	2082938..2083708	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
GBR75_RS10220	2083683..2084162	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
GBR75_RS10225	2084164..2084490	-	327	WP_001052491.1	anti-sigma factor antagonist	-
GBR75_RS10230	2084609..2085610	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
GBR75_RS10235	2085959..2086321	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GBR75_RS10240	2086318..2086488	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
GBR75_RS10245	2086573..2087721	-	1149	WP_001280702.1	alanine racemase	-
GBR75_RS10250	2087787..2088146	-	360	WP_000581194.1	holo-ACP synthase	-
GBR75_RS10255	2088150..2088641	-	492	WP_001205915.1	PH domain-containing protein	-
GBR75_RS10260	2088628..2090211	-	1584	WP_001294642.1	PH domain-containing protein	-
GBR75_RS10265	2090204..2090683	-	480	WP_001287083.1	hypothetical protein	-
GBR75_RS10270	2090892..2091452	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T141898 WP_000621175.1 NZ_CP045468:c2086321-2085959 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T141898 NZ_CP060160:951244-951456 [Staphylococcus lugdunensis]
GTGGAAAAGCGATTAATTTATATTACAAATGTGACTAATGAAGACGACTTGGAAAACTTACGAACAAGGTTATTAGAGAT
GATTGGTGTTAATGAAGTAGTGATCAATTTTGATGAACGTGCAGTTAATGTTACTTTCGAAACATCAACTAATTTAAATG
CACTTGAAAAAGAAATATATGATGCTGGCTATCATGCTATAGATGATTTATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT141898 WP_000948331.1 NZ_CP045468:c2086488-2086318 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT141898 NZ_CP060160:950938-951231 [Staphylococcus lugdunensis]
TTGAACGAACAAGATCATGCACATCATTCTAAAATTGTAAAAGCAAATTTAAAATCTAGATTAAATCGTATAGAAGGACA
AGTGCGGGCAATTAATCGCATGGTTGAAGATGATGTTTATTGTGATGATGTGTTAACACAAATAAGAGCAACACGCTCAG
CATTAACGAGTGTAGCAACAAAACTATTAGAGCATCATATGAAAAGTTGTATCATGGATAAAATAAACGATGGGCAGGAA
GTTGAAGCGATGGAGGAATTGCTAGTTACATTTCAAAAGATGCTAAAAGACTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1