Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4463304..4463801 Replicon chromosome
Accession NZ_CP045457
Organism Escherichia coli strain ET12567

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECET_RS21610 Protein ID WP_001173343.1
Coordinates 4463628..4463801 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECET_RS21605 Protein ID WP_000398619.1
Coordinates 4463304..4463600 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECET_RS21585 4460035..4460754 - 720 WP_000611288.1 two-component system response regulator DcuR -
ECET_RS21590 4460751..4462382 - 1632 WP_001216477.1 sensor histidine kinase -
ECET_RS21595 4462563..4462793 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
ECET_RS21600 4462805..4463077 + 273 WP_000405651.1 N-acetyltransferase -
ECET_RS21605 4463304..4463600 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECET_RS21610 4463628..4463801 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECET_RS21615 4463920..4465437 - 1518 WP_001295074.1 lysine--tRNA ligase -
ECET_RS21620 4465674..4467131 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T141882 WP_001173343.1 NZ_CP045457:4463628-4463801 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T141882 NZ_CP060141:c1818501-1818406 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT141882 WP_000398619.1 NZ_CP045457:4463304-4463600 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT141882 NZ_CP060141:1818321-1818378 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References