Detailed information of TA system TA141875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 2832199..2833636
• Replicon: chromosome
• Accession: NZ_CP045457
• Organism: Escherichia coli strain ET12567

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: ECET_RS13815
• Protein ID: WP_000155570.1
• Coordinates: 2832199..2833272 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: ECET_RS13820
• Protein ID: WP_000461704.1
• Coordinates: 2833265..2833636 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECET_RS13800	2827632..2829821	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
ECET_RS13805	2829818..2831434	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
ECET_RS13810	2831794..2832057	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
ECET_RS13815	2832199..2833272	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
ECET_RS13820	2833265..2833636	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
ECET_RS13825	2833991..2834854	+	864	WP_000787597.1	GTPase family protein	-
ECET_RS13830	2834946..2835767	+	822	WP_000197391.1	DUF945 domain-containing protein	-
ECET_RS13835	2835984..2836685	+	702	WP_000189409.1	WYL domain-containing protein	-
ECET_RS13840	2836726..2836962	+	237	WP_001144029.1	hypothetical protein	-
ECET_RS13845	2836962..2837405	+	444	WP_000824222.1	hypothetical protein	-
ECET_RS13850	2837429..2837896	+	468	WP_000702495.1	hypothetical protein	-
ECET_RS13855	2838121..2838435	-	315	WP_000065803.1	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T141875 WP_000155570.1 NZ_CP045457:2832199-2833272 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T141875 NZ_CP060141:1210825-1211091 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT141875 WP_000461704.1 NZ_CP045457:2833265-2833636 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT141875 NZ_CP060141:1210574-1210825 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• PDB: 6Y2P
• AlphaFold DB: A0A373F4G4