Detailed information of TA system TA141860

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 267227..267906
• Replicon: chromosome
• Accession: NZ_CP045457
• Organism: Escherichia coli strain ET12567

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: ECET_RS01255
• Protein ID: WP_000854672.1
• Coordinates: 267227..267568 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: ECET_RS01260
• Protein ID: WP_000070395.1
• Coordinates: 267589..267906 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECET_RS01230	262504..262905	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
ECET_RS01235	262944..263999	-	1056	WP_000749863.1	phosphoporin PhoE	-
ECET_RS01240	264287..265390	+	1104	WP_001285288.1	glutamate 5-kinase	-
ECET_RS01245	265402..266655	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
ECET_RS01255	267227..267568	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
ECET_RS01260	267589..267906	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
ECET_RS01265	267925..268146	-	222	WP_000691994.1	DUF987 domain-containing protein	-
ECET_RS01270	268155..268631	-	477	WP_000811693.1	RadC family protein	-
ECET_RS01275	268647..269105	-	459	WP_000211838.1	antirestriction protein	-
ECET_RS01280	269203..269442	-	240	WP_000194654.1	DUF905 domain-containing protein	-
ECET_RS01285	269519..269986	-	468	WP_001547765.1	hypothetical protein	-
ECET_RS01290	270009..270452	-	444	WP_000824223.1	hypothetical protein	-
ECET_RS01295	270452..270679	-	228	WP_001548158.1	hypothetical protein	-
ECET_RS01300	270675..270866	-	192	Protein_253	DeoR family transcriptional regulator	-
ECET_RS01305	271083..271904	-	822	WP_000197389.1	DUF945 domain-containing protein	-
ECET_RS01310	271996..272859	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	267007..285870	18863

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T141860 WP_000854672.1 NZ_CP045457:c267568-267227 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T141860 NZ_CP060125:4926-5300 [Sphingobium yanoikuyae]
ATGGATACGGAACGGCCGGTTCGATGGGTGGCATCGTCAAAGCGCGATTTTCGGGAGTTCCCGGACGATGTGCAGGACGT
AATGGGCTATGCCCTGCACCTTGCGCAGCAAGGCGGGCAGCACGCCAGCACTAAGCCGTTGAAGGGCTTTGGCGGCGCTG
GCGTGGTCGAGATCATAGACGACCACCAAGGCGATACGTTCCGCACGGTCTATACGGTCAAATTCGCGGAGGCGGTCTAT
GTCCTGCACGCCTTCCAGAAGAAATCCAAGCAGGGCAAGGCCACGCCGCAGGCCGATATGGATTTGATCCGAACGCGGCT
GAAATCCGCCGAGGAGCATCACCGGCAACACCAGACCCCGCGAGGCACAGCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT141860 WP_000070395.1 NZ_CP045457:c267906-267589 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT141860 NZ_CP060125:5297-5623 [Sphingobium yanoikuyae]
ATGAGCGCACAGGACGAAATCATTGAGGAAGGCAGCGGCAATGTCTTTGCCGATCTAGGCTTTGCCGACCCCGACACCCA
CCTTTTGAAAGCCCAGCTCGTTACCCGTGTAGCCGAGGCCATGCAGGAACGTAAATTGACGCAGATCGCCGCCGCCAAGG
TTACGGGATCTACCCAACCGGAATTGTCGCGCATCCTGCGAGGCCAGTTCCGCAGCGTCTCGGTTGAGCGGCTTCTGGCC
ATGCTCACCCGGCTTGGCTGTAAGGTCGATATTGTCGTGCGTCCGCAGGGGCGGACCACTGAATCGGCCGTGATCCACTT
CGCCTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684