Detailed information of TA system TA141856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-PrlF
• Location: 3179223..3179760
• Replicon: chromosome
• Accession: NZ_CP045456
• Organism: Brevundimonas sp. Bb-A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BSP_RS15705
• Protein ID: WP_152950956.1
• Coordinates: 3179425..3179760 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BSP_RS15700
• Protein ID: WP_152950955.1
• Coordinates: 3179223..3179438 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSP_RS15685	3175904..3176683	-	780	WP_194167038.1	ZIP family metal transporter	-
BSP_RS15690	3176751..3177908	+	1158	WP_152950953.1	glutamate 5-kinase	-
BSP_RS15695	3177905..3179176	+	1272	WP_152950954.1	glutamate-5-semialdehyde dehydrogenase	-
BSP_RS15700	3179223..3179438	+	216	WP_152950955.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BSP_RS15705	3179425..3179760	+	336	WP_152950956.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BSP_RS15710	3179819..3182242	-	2424	WP_152950957.1	membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family	-
BSP_RS15715	3182472..3183899	+	1428	WP_152950958.1	NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-108)

Antitoxin

• PrlF antitoxin (4-52)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12359.23 Da
• Isoelectric Point: 9.7716

>T141856 WP_152950956.1 NZ_CP045456:3179425-3179760 [Brevundimonas sp. Bb-A]
MPDFERGDVVRVPFPYTDRNTRQHRPALVVSNGPLGESDRLLWVVMITSAENRRWAEDVPLDEDYAAAGLPAPSLIRPVK
IATIDAAVAKKLGRIDPDRLARAMQSVRAKL

Nucleotide

• Length: 336 bp

>T141856 NZ_CP060122:c4508255-4507824 [Sphingobium yanoikuyae]
ATGAGTTTCCTGCTCGATACCAACATCATCTCGGAGGGAGCAAAGCCGCGTCCTGATCCCGGCGTGATGGATTGGCTGGC
GTCGGTGGATGAGGAAGAGCTGTTCCTGAGCGTGGTATCGCTGGCAGAACTGCGCCACGGCATTGAGCGCATGGACAAGG
GGGCACGACAGTCGGGCCTCGACATCTGGCTGACCGAACAACTGGCGCCGCGATTTGAGGAAAGACTGCTCTCCATCGAC
AGCGTAACGGCGGATCTGTGGGGCCGTATTGTCAATCGAGCACAAGGCGCGGGCCGCCCCATGGGAGCGATGGATGCTTT
CATCGCCGCCCATGCGCAGCAGCACGAACTCACACTCGTGACCCGCAATATTTCCGACTTCGAGGCGACTGGTATCCGCC
TGTTCAACCCATGGACCGAAGGGAAAGCCTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7998.88 Da
• Isoelectric Point: 4.4949

>AT141856 WP_152950955.1 NZ_CP045456:3179223-3179438 [Brevundimonas sp. Bb-A]
MIKSRLTSKAQTTIPQAVRTALNLQDGDEIQYDLDGDRAILRKAWRAPADDPFSAFDEWDTEADRKAYAGL

Nucleotide

• Length: 216 bp

>AT141856 NZ_CP060122:c4508521-4508252 [Sphingobium yanoikuyae]
ATGGCAGTCGAAACGCATCCGGATAGCTGGACGGTAGCACAGGCCAAGGCGCGCCTGAGCGAGGTAATCGAGAAGGCGCG
GCGCGAGCCCCAGGCGATTACGCGTCACGGCAAAAGCGCCGTCGTCGTGGTCGATGCCGAACTATGGGAACGGGTTCAAC
GTCGCCAGCATCGCGGCAGCTTCGCCGACTTCCTGCTGACCTCGCCGTTGCGCGGATCGGGTCTTGAGGTCGATCGCCTC
AAGGATGGTGTTCGCGAGATTGATCTATGA