Detailed information of TA system TA141835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 609936..610512
• Replicon: chromosome
• Accession: NZ_CP045451
• Organism: Enterobacter hormaechei strain 14320055602

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A800YKM1
• Locus tag: GEA57_RS02925
• Protein ID: WP_015572580.1
• Coordinates: 609936..610223 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A801DSF4
• Locus tag: GEA57_RS02930
• Protein ID: WP_017694570.1
• Coordinates: 610210..610512 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEA57_RS02900	605053..605748	+	696	WP_203436092.1	winged helix-turn-helix domain-containing protein	-
GEA57_RS02905	605898..606368	+	471	WP_032647140.1	MarR family transcriptional regulator	-
GEA57_RS02910	606365..607432	+	1068	WP_026094404.1	HlyD family secretion protein	-
GEA57_RS02915	607422..608498	+	1077	WP_015572578.1	DUF2955 domain-containing protein	-
GEA57_RS02920	608495..609766	-	1272	WP_015572579.1	DUF445 domain-containing protein	-
GEA57_RS02925	609936..610223	+	288	WP_015572580.1	BrnT family toxin	Toxin
GEA57_RS02930	610210..610512	+	303	WP_017694570.1	BrnA antitoxin family protein	Antitoxin
GEA57_RS02935	610541..611179	-	639	WP_203436093.1	LysE family translocator	-
GEA57_RS02940	611218..611970	-	753	WP_203436094.1	AraC family transcriptional regulator	-
GEA57_RS02945	612124..613494	+	1371	WP_017694568.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
GEA57_RS02950	613676..614221	-	546	WP_006810254.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11296.81 Da
• Isoelectric Point: 7.3587

>T141835 WP_015572580.1 NZ_CP045451:609936-610223 [Enterobacter hormaechei]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIRIIS
ARKADRKERRRYEHR

Nucleotide

• Length: 288 bp

>T141835 NZ_CP060121:c1368260-1368066 [Escherichia coli BL21]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11482.13 Da
• Isoelectric Point: 10.4094

>AT141835 WP_017694570.1 NZ_CP045451:610210-610512 [Enterobacter hormaechei]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT141835 NZ_CP060121:1367889-1368067 [Escherichia coli BL21]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT