Detailed information of TA system TA141819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2302242..2302981
• Replicon: chromosome
• Accession: NZ_CP045450
• Organism: Enterobacter hormaechei strain 14280161202

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: GEA58_RS10965
• Protein ID: WP_203453353.1
• Coordinates: 2302242..2302727 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: GEA58_RS10970
• Protein ID: WP_003857131.1
• Coordinates: 2302715..2302981 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEA58_RS10935	2297260..2298018	+	759	WP_045341969.1	DeoR/GlpR family DNA-binding transcription regulator	-
GEA58_RS10940	2298123..2299415	+	1293	WP_203453351.1	glycoside hydrolase	-
GEA58_RS10945	2299484..2300029	+	546	WP_032671141.1	NUDIX hydrolase	-
GEA58_RS10950	2300213..2300848	-	636	WP_203453352.1	DUF421 domain-containing protein	-
GEA58_RS10955	2300858..2301304	-	447	WP_014069654.1	DUF3290 domain-containing protein	-
GEA58_RS10960	2301733..2302061	-	329	Protein_2136	helix-turn-helix domain-containing protein	-
GEA58_RS10965	2302242..2302727	-	486	WP_203453353.1	GNAT family N-acetyltransferase	Toxin
GEA58_RS10970	2302715..2302981	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
GEA58_RS10975	2303045..2303974	-	930	WP_193360842.1	LysR family transcriptional regulator	-
GEA58_RS10980	2304104..2305486	+	1383	WP_203453354.1	MFS transporter	-
GEA58_RS10985	2305508..2306503	-	996	WP_045339807.1	DUF2891 domain-containing protein	-
GEA58_RS10990	2306513..2307499	-	987	WP_128771619.1	DUF979 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2286837..2302981	16144

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17497.20 Da
• Isoelectric Point: 9.6283

>T141819 WP_203453353.1 NZ_CP045450:c2302727-2302242 [Enterobacter hormaechei]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLLGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T141819 NZ_CP060117:c3239842-3239588 [Shigella sonnei]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT141819 WP_003857131.1 NZ_CP045450:c2302981-2302715 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT141819 NZ_CP060117:c3240090-3239839 [Shigella sonnei]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9