Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2232667..2233196 Replicon chromosome
Accession NZ_CP045435
Organism Staphylococcus aureus strain 08-028

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag SA08028_RS11335 Protein ID WP_000621175.1
Coordinates 2232667..2233029 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag SA08028_RS11340 Protein ID WP_000948331.1
Coordinates 2233026..2233196 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SA08028_RS11315 2229645..2230415 - 771 WP_001041108.1 RNA polymerase sigma factor SigB -
SA08028_RS11320 2230390..2230869 - 480 WP_001190830.1 anti-sigma B factor RsbW -
SA08028_RS11325 2230871..2231197 - 327 WP_001052491.1 anti-sigma factor antagonist -
SA08028_RS11330 2231316..2232317 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
SA08028_RS11335 2232667..2233029 - 363 WP_000621175.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
SA08028_RS11340 2233026..2233196 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
SA08028_RS11345 2233281..2234429 - 1149 WP_075111630.1 alanine racemase -
SA08028_RS11350 2234495..2234854 - 360 WP_000581197.1 holo-ACP synthase -
SA08028_RS11355 2234858..2235349 - 492 WP_001205912.1 PH domain-containing protein -
SA08028_RS11360 2235336..2236919 - 1584 WP_001294626.1 PH domain-containing protein -
SA08028_RS11365 2236912..2237391 - 480 WP_001287078.1 hypothetical protein -
SA08028_RS11370 2237600..2238160 - 561 WP_001092409.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13441.69 Da        Isoelectric Point: 10.1654

>T141804 WP_000621175.1 NZ_CP045435:c2233029-2232667 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Download         Length: 363 bp

>T141804 NZ_CP060111:2230408-2230511 [Klebsiella michiganensis]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT141804 WP_000948331.1 NZ_CP045435:c2233196-2233026 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT141804 NZ_CP060111:c2230518-2230350 [Klebsiella michiganensis]
AACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGTCCGCGACAAAAGTG
GACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References