Detailed information of TA system TA141787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 1868949..1870542
• Replicon: chromosome
• Accession: NZ_CP045434
• Organism: Sphingomonas ginsengisoli An et al. 2013 strain KACC 16858

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: GCU42_RS08995
• Protein ID: WP_114227205.1
• Coordinates: 1869565..1870542 (+)
• Length: 326 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: GCU42_RS08990
• Protein ID: WP_114227204.1
• Coordinates: 1868949..1869572 (+)
• Length: 208 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU42_RS08980	1866078..1867070	-	993	WP_114227202.1	ATP-binding protein	-
GCU42_RS08985	1867388..1868521	-	1134	WP_114227203.1	DUF2235 domain-containing protein	-
GCU42_RS08990	1868949..1869572	+	624	WP_114227204.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
GCU42_RS08995	1869565..1870542	+	978	WP_114227205.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GCU42_RS09000	1870550..1871257	-	708	WP_152569530.1	protein phosphatase 2C domain-containing protein	-
GCU42_RS09005	1871494..1872198	-	705	WP_152569531.1	hypothetical protein	-
GCU42_RS09010	1872271..1872960	-	690	WP_114227207.1	hypothetical protein	-
GCU42_RS09015	1872953..1873198	-	246	WP_152569532.1	hypothetical protein	-
GCU42_RS09020	1873507..1874430	+	924	WP_152569533.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1863472..1918043	54571

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-265)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (11-57)

Sequences

Toxin

Protein

• Length: 326 a.a.
• Molecular weight: 35597.19 Da
• Isoelectric Point: 4.6870

>T141787 WP_114227205.1 NZ_CP045434:1869565-1870542 [Sphingomonas ginsengisoli An et al. 2013]
MAKDIKDVGTSVRARLLSLSRKTGQANELLLTRYALERLLYRMGMTEHASRFVLKGAMLMTTWFDDPHRPTRDIDLLCFG
DPRPDEMLQVFREICSIQEKDGVEFDVDSLGVTANREDLAYGGLRLQTYATISGARLRIIIDIGFGDSTEPGLEELELPV
LLDQPQPHLRAYARETVVAEKFQAMVMLGQANSRLKDYYDLWLLARSYEFDPHRLAGAIAATFRRRGTPIPTDVPDGLKR
EFYEDRAKTSQWRAFVDDVAVDPGSLADVAAALSDFLMPAARAGASLTDGGADGSAAGGGSDPPANSADDDGAPPTLVQS
GTRPS

Nucleotide

• Length: 978 bp

>T141787 NZ_CP060088:2007755-2008033 [Vibrio parahaemolyticus]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTTGAGTTTCTCTATGACTTCAACCCTGATGCTGC
AGAAAAAACTGACAACCTCATTGAAGCAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGTGTCCAACGAGATGGCA
TCCGCGGACGATTACTCATCATTCCTGAGATTTCGATGATTGTCTCTTACTGGATTGAGGACGATATCATCCGAGTTATG
CGTGTACTACACCAGAAACAAAAATTCCCTACGGATTGA

Antitoxin

Protein

• Length: 208 a.a.
• Molecular weight: 23230.77 Da
• Isoelectric Point: 10.0925

>AT141787 WP_114227204.1 NZ_CP045434:1868949-1869572 [Sphingomonas ginsengisoli An et al. 2013]
MSEESTASHKERIVHLLQQQNAARMSEILAAGATATAVSRLERDGTIVRLSRGLYQLADRPLEANQPLIEAAKLVSKGVI
CLVSALSYHELTDQIPRRVSMAIGPRDWLPQITYPPIRFFRFSPDALTKHVETRTIDGVDIRVTNPARTIVDLFKYRGKV
GQNVAIDGLKEVFRQRRATPAEVHNIAAEMKQWKVMQPYVEALSFNG

Nucleotide

• Length: 624 bp

>AT141787 NZ_CP060088:2007474-2007758 [Vibrio parahaemolyticus]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCTGAGAGCCAAGGTCGCAC
TCTGAGTGACGCATGCCGTGAACTTACTGAGCAACTCGCTGAACAACAAAGAAAAACATTATCTCACGATGCATGGCTAA
CTGAACAAGTAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAATCCGTTTTCGTTGAGCACCAAACGGCTAAGTCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGTAATCGAGGTAAGCAATGA