Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 525032..525668 Replicon chromosome
Accession NZ_CP045425
Organism Bacillus subtilis strain JAAA

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GCU76_RS02680 Protein ID WP_003156187.1
Coordinates 525318..525668 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag GCU76_RS02675 Protein ID WP_003225183.1
Coordinates 525032..525313 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GCU76_RS02655 521392..521991 - 600 WP_017697004.1 rhomboid family intramembrane serine protease -
GCU76_RS02660 522086..522451 + 366 WP_014475874.1 holo-ACP synthase -
GCU76_RS02665 522617..523633 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
GCU76_RS02670 523747..524916 + 1170 WP_017697002.1 alanine racemase -
GCU76_RS02675 525032..525313 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GCU76_RS02680 525318..525668 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GCU76_RS02685 525783..526607 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
GCU76_RS02690 526612..526977 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
GCU76_RS02695 526981..527382 + 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
GCU76_RS02700 527394..528401 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
GCU76_RS02705 528463..528792 + 330 WP_017697000.1 anti-sigma factor antagonist RsbV -
GCU76_RS02710 528789..529271 + 483 WP_003234299.1 anti-sigma B factor RsbW -
GCU76_RS02715 529237..530025 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
GCU76_RS02720 530025..530624 + 600 WP_017696999.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T141774 WP_003156187.1 NZ_CP045425:525318-525668 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T141774 NZ_CP060084:3687202-3687501 [Teredinibacter haidensis]
ATGAGCTATAGCCTCGAATTTAAAAAGAGTGCTTTAAAAGAATGGAAAAAGCTCGCCCCATCAATACGACAAGTATTTAA
AAAGAAACTAACCGAACGACTTGAAAACCCTCATGTGCTTTCCGCTGCTGTTAGCGGCGGGAATAACTTATACAAAATTA
AATTACGCCAACTTGGCTACCGCTTAGTATATTCAGTTTCCGATACAACAATTACAGTGACCGTTCTTGCTGCAGGAAAA
AGAAACCGTAACGAAGTCTATGATTTGGCGCTTTCTCGTGATGACCCCGACAAGCTATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT141774 WP_003225183.1 NZ_CP045425:525032-525313 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT141774 NZ_CP060084:3686964-3687212 [Teredinibacter haidensis]
ATGCCAAATCAAATACTTACCGAAATGGCTGCAAGCATTTCTGAGCTAAAAGCCAACCCCATGAAGGTTGTTGCTAGCGG
TGAAGGTATGCCAATAGCCGTGCTAAACAGGAACGAACCAGCCTTTTATTGCATCCCAGCTAAAGCCTACGAGGCTATGA
TGGACCTCATAGAAGATAAAGACCTTCTAGAGGTAGCCAGAAGCCGCATGGATGAAGAAAGCGTAAAGGTGAAACTGGAT
GAGCTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References