Detailed information of TA system TA141769

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 14594..16226
• Replicon: plasmid pTHAF9_c
• Accession: NZ_CP045407
• Organism: Roseovarius sp. THAF9

Toxin (Protein)

• Gene name: HipA3
• Uniprot ID: -
• Locus tag: FIU86_RS21615
• Protein ID: WP_152477446.1
• Coordinates: 14925..16226 (+)
• Length: 434 a.a.

Antitoxin (Protein)

• Gene name: HipB3
• Uniprot ID: A0A196Q0Q5
• Locus tag: FIU86_RS21610
• Protein ID: WP_064225030.1
• Coordinates: 14594..14932 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU86_RS21595	9768..11729	+	1962	WP_152477444.1	ATP-dependent helicase	-
FIU86_RS21600	11743..12834	-	1092	WP_152477445.1	beta family protein	-
FIU86_RS21605	12803..14131	-	1329	WP_064225031.1	ImmA/IrrE family metallo-endopeptidase	-
FIU86_RS21610	14594..14932	+	339	WP_064225030.1	helix-turn-helix domain-containing protein	Antitoxin
FIU86_RS21615	14925..16226	+	1302	WP_152477446.1	type II toxin-antitoxin system HipA family toxin	Toxin
FIU86_RS21620	16305..16952	-	648	Protein_14	transposase	-
FIU86_RS21625	17290..18681	-	1392	WP_152477447.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
FIU86_RS21630	18777..19871	-	1095	WP_152477448.1	alkene reductase	-
FIU86_RS21635	20061..21047	-	987	WP_152477449.1	aldo/keto reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89971	89971
	-	flank	IS/Tn	-	-	16305..16982	677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-115)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 434 a.a.
• Molecular weight: 48507.60 Da
• Isoelectric Point: 6.0817

>T141769 WP_152477446.1 NZ_CP045407:14925-16226 [Roseovarius sp. THAF9]
MTDASIVLWGRRIGAVSWDEARALGIFQYDPAFVGAGIEVAPLKMPVREAPYEFPALPKETFKGLPGMLADALPDKFGNR
LIDAWLAETGRSADRFSPVDRLCYIGNRGIGALEFEPTVRKATRSKKLEVAQLVDLANRVLSERENLAGRLGGEDDAEAL
EDILSVGTSAGGARAKAVLAWNRDTGEFRSGQVKVNEGYEHWLLKFDGVSNNRDKELADPQGFGKIEYAYYLMATEAGID
MSECRLHHEGGRSHFMTRRFDRDERGRKRHMQSLGAMQHFDFNDPASYSYEQAVLTIRDLGLGMDVVEQQYKRAVFNVVA
RNQDDHVKNISFLMDRSGAWRLSPAYDVAYSYNPSGSWTRDHQMSLAGKRNDFTHGDLMVLASNVGLKSNKANQAIEEIV
DAVAKWQEFAEQAGVEYSDMSRIEKTFRMNLRG

Nucleotide

• Length: 1302 bp

>T141769 NZ_CP060083:c4427462-4427175 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTGGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCG
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGTCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12568.13 Da
• Isoelectric Point: 6.4178

>AT141769 WP_064225030.1 NZ_CP045407:14594-14932 [Roseovarius sp. THAF9]
MATSQQIEQEIGQRLARLRLARNVTQTDLAEKAGIGVRTLRRLEAGDPSTLDTFLRVATALDLEEPILSALPEGDIRPIE
RVSQKGKERRRASPVSERSRKSTWTWGDDVDD

Nucleotide

• Length: 339 bp

>AT141769 NZ_CP060083:c4427188-4426886 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAATTGAAGGCGCTGGCGAA
TAAATCCGACGAAGAGATTGATTACAGCGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A196Q0Q5