Detailed information of TA system TA141768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 293514..294150
• Replicon: chromosome
• Accession: NZ_CP045403
• Organism: Bacillus sp. THAF10

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0K6GQX7
• Locus tag: FIU87_RS01575
• Protein ID: WP_010195163.1
• Coordinates: 293800..294150 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: FIU87_RS01570
• Protein ID: WP_152442974.1
• Coordinates: 293514..293795 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU87_RS01545	289535..290248	-	714	WP_152442970.1	rhomboid family intramembrane serine protease	-
FIU87_RS01550	290356..290715	+	360	WP_152446370.1	holo-ACP synthase	-
FIU87_RS01555	290884..291903	+	1020	WP_172970910.1	outer membrane lipoprotein carrier protein LolA	-
FIU87_RS01560	291944..292132	+	189	WP_152442972.1	hypothetical protein	-
FIU87_RS01565	292196..293371	+	1176	WP_152442973.1	alanine racemase	-
FIU87_RS01570	293514..293795	+	282	WP_152442974.1	antitoxin endoai	Antitoxin
FIU87_RS01575	293800..294150	+	351	WP_010195163.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
FIU87_RS01580	294400..295227	+	828	WP_152442975.1	STAS domain-containing protein	-
FIU87_RS01585	295224..295580	+	357	WP_047972208.1	STAS domain-containing protein	-
FIU87_RS01590	295584..295985	+	402	WP_152442976.1	anti-sigma regulatory factor	-
FIU87_RS01595	296001..297008	+	1008	WP_152442977.1	PP2C family protein-serine/threonine phosphatase	-
FIU87_RS01600	297084..297392	+	309	WP_152442978.1	STAS domain-containing protein	-
FIU87_RS01605	297450..297926	+	477	WP_152442979.1	anti-sigma B factor RsbW	-
FIU87_RS01610	297901..298686	+	786	WP_152442980.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13006.03 Da
• Isoelectric Point: 4.8891

>T141768 WP_010195163.1 NZ_CP045403:293800-294150 [Bacillus sp. THAF10]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDEEMMDKVDEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T141768 NZ_CP060083:4089275-4089553 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10703.18 Da
• Isoelectric Point: 7.0156

>AT141768 WP_152442974.1 NZ_CP045403:293514-293795 [Bacillus sp. THAF10]
VSESSATTEILIRLPQNLVSELDGLVKQENGNRNDLIFQATKMYLRERKKRQIRESMRRGYMEMSKINLNIASEAFLAEY
EAEHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT141768 NZ_CP060083:4089012-4089272 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACACGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0K6GQX7