Detailed information of TA system TA141762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 18856..19321
• Replicon: plasmid pTHAF27_c
• Accession: NZ_CP045396
• Organism: Roseovarius sp. THAF27

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: FIU89_RS22025
• Protein ID: WP_152494733.1
• Coordinates: 18856..19119 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: FIU89_RS22030
• Protein ID: WP_152494734.1
• Coordinates: 19106..19321 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU89_RS21985	14000..15064	+	1065	WP_152494726.1	replication initiator protein A	-
FIU89_RS21990	15061..15375	-	315	WP_152494727.1	hypothetical protein	-
FIU89_RS21995	15372..16100	-	729	WP_152494728.1	ParA family protein	-
FIU89_RS22000	16249..16515	-	267	WP_152494729.1	Txe/YoeB family addiction module toxin	-
FIU89_RS22005	16512..16763	-	252	WP_036743750.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
FIU89_RS22010	16870..17556	-	687	WP_152494730.1	helix-turn-helix transcriptional regulator	-
FIU89_RS22015	17811..18440	+	630	WP_172978219.1	tyrosine-type recombinase/integrase	-
FIU89_RS22020	18440..18691	+	252	WP_152494732.1	WGR domain-containing protein	-
FIU89_RS22025	18856..19119	+	264	WP_152494733.1	BrnT family toxin	Toxin
FIU89_RS22030	19106..19321	+	216	WP_152494734.1	CopG family transcriptional regulator	Antitoxin
FIU89_RS22035	19465..20013	+	549	Protein_23	IS5 family transposase	-
FIU89_RS22040	19997..20854	-	858	Protein_24	IS5 family transposase	-
FIU89_RS22045	21169..22611	+	1443	WP_037239070.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
FIU89_RS22050	22670..23704	+	1035	WP_152494735.1	TRAP transporter substrate-binding protein DctP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..23734	23734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10188.27 Da
• Isoelectric Point: 4.8616

>T141762 WP_152494733.1 NZ_CP045396:18856-19119 [Roseovarius sp. THAF27]
MKFEYDPDKSAANREKHGIDFDEAQALWDDPYLIEAPANVTDEPRFLAVGMIGARHWTAVYTYRSDRVRIISVRRARTQE
IDHYESD

Nucleotide

• Length: 264 bp

>T141762 NZ_CP060083:2601831-2602118 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8216.45 Da
• Isoelectric Point: 8.8085

>AT141762 WP_152494734.1 NZ_CP045396:19106-19321 [Roseovarius sp. THAF27]
MKATEFDERFAAGEDMSAHVDWTKARRLNVEAKRVNVDFPTWVVAGLDRQAQKLGITRQALIKMWIAERLE

Nucleotide

• Length: 216 bp

>AT141762 NZ_CP060083:2601592-2601831 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA