Detailed information of TA system TA141750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 121204..121835
• Replicon: plasmid pTHAF40_a
• Accession: NZ_CP045361
• Organism: Roseivivax sp. THAF40

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FIU97_RS19025
• Protein ID: WP_152456043.1
• Coordinates: 121204..121605 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FIU97_RS19030
• Protein ID: WP_152452774.1
• Coordinates: 121605..121835 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU97_RS19000	116312..118378	-	2067	WP_152456086.1	polysaccharide biosynthesis tyrosine autokinase	-
FIU97_RS19005	118618..118956	-	339	WP_152456038.1	hypothetical protein	-
FIU97_RS19010	119407..120513	+	1107	WP_172976596.1	glycosyltransferase	-
FIU97_RS19015	120687..120866	+	180	WP_152456041.1	hypothetical protein	-
FIU97_RS19020	120878..121104	-	227	Protein_96	type II toxin-antitoxin system ParD family antitoxin	-
FIU97_RS19025	121204..121605	-	402	WP_152456043.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FIU97_RS19030	121605..121835	-	231	WP_152452774.1	antitoxin	Antitoxin
FIU97_RS19035	122331..122876	-	546	WP_152456045.1	hypothetical protein	-
FIU97_RS19040	122869..123402	-	534	WP_152456047.1	hypothetical protein	-
FIU97_RS19045	123816..124064	-	249	WP_172976597.1	helix-turn-helix domain-containing protein	-
FIU97_RS19050	124184..125020	+	837	WP_152456049.1	tyrosine-type recombinase/integrase	-
FIU97_RS19055	125355..125939	-	585	WP_152456051.1	terminase small subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fcl / gmd	1..219612	219612

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14637.81 Da
• Isoelectric Point: 6.8624

>T141750 WP_152456043.1 NZ_CP045361:c121605-121204 [Roseivivax sp. THAF40]
MLRYMLDTNILIYTIKNKPEAVRKQLEAHDGEMCASSLTAMELLYGAHKSQSVRRNLDVIEGLLSRIDVLDFDLSAAEHA
GQIRAELAASGTSIGPFDVMIAGHARSRGMCLITNNEGEFSRVSGLRLGTWTK

Nucleotide

• Length: 402 bp

>T141750 NZ_CP060083:180455-180562 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8700.70 Da
• Isoelectric Point: 4.7708

>AT141750 WP_152452774.1 NZ_CP045361:c121835-121605 [Roseivivax sp. THAF40]
MERGSVFMTNKTQAVRFPKSVRLPEGVTSVDIERRGRSWVITPSEQGWAEWFSGPEVSQDFMSEREQPADQERAAL

Nucleotide

• Length: 231 bp

>AT141750 NZ_CP060083:c180406-180343 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT