Detailed information of TA system TA141746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-StbC
• Location: 1142726..1143387
• Replicon: chromosome
• Accession: NZ_CP045359
• Organism: Pseudomonas sp. THAF42

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FIU98_RS05230
• Protein ID: WP_152488000.1
• Coordinates: 1142726..1143145 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FIU98_RS05235
• Protein ID: WP_152488001.1
• Coordinates: 1143142..1143387 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU98_RS05205	1139578..1140492	+	915	WP_152487997.1	DMT family transporter	-
FIU98_RS05210	1140551..1140997	-	447	WP_152487998.1	hypothetical protein	-
FIU98_RS24780	1141025..1141198	-	174	WP_172979130.1	hypothetical protein	-
FIU98_RS05220	1141606..1142181	+	576	WP_152487999.1	hypothetical protein	-
FIU98_RS05225	1142330..1142707	-	378	WP_172979131.1	excalibur calcium-binding domain-containing protein	-
FIU98_RS05230	1142726..1143145	-	420	WP_152488000.1	type II toxin-antitoxin system VapC family toxin	Toxin
FIU98_RS05235	1143142..1143387	-	246	WP_152488001.1	Arc family DNA-binding protein	Antitoxin
FIU98_RS05240	1143576..1143944	-	369	WP_152488002.1	hypothetical protein	-
FIU98_RS05245	1144062..1144814	+	753	WP_152488003.1	class I SAM-dependent methyltransferase	-
FIU98_RS05250	1144925..1145380	+	456	WP_152488004.1	MerR family transcriptional regulator	-
FIU98_RS05255	1145328..1145714	+	387	WP_152488005.1	carboxymuconolactone decarboxylase family protein	-
FIU98_RS05260	1146928..1147791	+	864	Protein_1043	integrase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1141606..1166733	25127

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (13-45)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15121.40 Da
• Isoelectric Point: 4.2953

>T141746 WP_152488000.1 NZ_CP045359:c1143145-1142726 [Pseudomonas sp. THAF42]
MILLDTNVLSELMRAKPAPQVLEWVDAQPVGDLVITSITVAEILYGIARMPDGKRKQGLLDVASAMFDEDFAGNILPFDA
DAAVYYAEIAAETEAKRRVVDMADAQIAAIGRLHDAVIATRNIRHFETLGVALVDPWSA

Nucleotide

• Length: 420 bp

>T141746 NZ_CP060075:3925906-3926184 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTACACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8841.99 Da
• Isoelectric Point: 10.4369

>AT141746 WP_152488001.1 NZ_CP045359:c1143387-1143142 [Pseudomonas sp. THAF42]
MAAITIRNLDDDLKAQLRIVAASHGRSMEEEARVIIRQALSRQGKRGGLGSRIHSRFAAVGGADLELPERKDNARAASFD
E

Nucleotide

• Length: 246 bp

>AT141746 NZ_CP060075:3925643-3925903 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA