Detailed information of TA system TA141729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-StbC
• Location: 1142734..1143395
• Replicon: chromosome
• Accession: NZ_CP045349
• Organism: Pseudomonas sp. THAF187a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FIV02_RS05230
• Protein ID: WP_152488000.1
• Coordinates: 1142734..1143153 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FIV02_RS05235
• Protein ID: WP_152488001.1
• Coordinates: 1143150..1143395 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIV02_RS05205	1139586..1140500	+	915	WP_152487997.1	DMT family transporter	-
FIV02_RS05210	1140559..1141005	-	447	WP_152487998.1	hypothetical protein	-
FIV02_RS24780	1141033..1141206	-	174	WP_172979130.1	hypothetical protein	-
FIV02_RS05220	1141614..1142189	+	576	WP_152487999.1	hypothetical protein	-
FIV02_RS05225	1142338..1142715	-	378	WP_172979131.1	excalibur calcium-binding domain-containing protein	-
FIV02_RS05230	1142734..1143153	-	420	WP_152488000.1	type II toxin-antitoxin system VapC family toxin	Toxin
FIV02_RS05235	1143150..1143395	-	246	WP_152488001.1	Arc family DNA-binding protein	Antitoxin
FIV02_RS05240	1143584..1143952	-	369	WP_152488002.1	hypothetical protein	-
FIV02_RS05245	1144070..1144822	+	753	WP_152488003.1	class I SAM-dependent methyltransferase	-
FIV02_RS05250	1144933..1145388	+	456	WP_152488004.1	MerR family transcriptional regulator	-
FIV02_RS05255	1145336..1145722	+	387	WP_152488005.1	carboxymuconolactone decarboxylase family protein	-
FIV02_RS05260	1146936..1147799	+	864	Protein_1043	integrase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1141614..1166741	25127

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (13-45)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15121.40 Da
• Isoelectric Point: 4.2953

>T141729 WP_152488000.1 NZ_CP045349:c1143153-1142734 [Pseudomonas sp. THAF187a]
MILLDTNVLSELMRAKPAPQVLEWVDAQPVGDLVITSITVAEILYGIARMPDGKRKQGLLDVASAMFDEDFAGNILPFDA
DAAVYYAEIAAETEAKRRVVDMADAQIAAIGRLHDAVIATRNIRHFETLGVALVDPWSA

Nucleotide

• Length: 420 bp

>T141729 NZ_CP060075:311292-311567 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8841.99 Da
• Isoelectric Point: 10.4369

>AT141729 WP_152488001.1 NZ_CP045349:c1143395-1143150 [Pseudomonas sp. THAF187a]
MAAITIRNLDDDLKAQLRIVAASHGRSMEEEARVIIRQALSRQGKRGGLGSRIHSRFAAVGGADLELPERKDNARAASFD
E

Nucleotide

• Length: 246 bp

>AT141729 NZ_CP060075:311564-311923 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA