Detailed information of TA system TA141724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 158436..159084
• Replicon: plasmid pTHAF190c_c
• Accession: NZ_CP045341
• Organism: Vibrio sp. THAF190c

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: FIV04_RS25970
• Protein ID: WP_152471335.1
• Coordinates: 158436..158837 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: FIV04_RS25975
• Protein ID: WP_152471312.1
• Coordinates: 158851..159084 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIV04_RS25950	153874..156060	+	2187	WP_152471308.1	conjugative transfer system coupling protein TraD	-
FIV04_RS27070	156613..156816	-	204	WP_172974342.1	hypothetical protein	-
FIV04_RS25955	157076..157531	+	456	WP_152471309.1	hypothetical protein	-
FIV04_RS25960	157536..157928	+	393	WP_152471310.1	hypothetical protein	-
FIV04_RS25965	158006..158275	+	270	WP_152471311.1	hypothetical protein	-
FIV04_RS25970	158436..158837	-	402	WP_152471335.1	type II toxin-antitoxin system VapC family toxin	Toxin
FIV04_RS25975	158851..159084	-	234	WP_152471312.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FIV04_RS25980	159235..159786	+	552	WP_152471313.1	hypothetical protein	-
FIV04_RS25985	159799..160143	+	345	WP_152471314.1	hypothetical protein	-
FIV04_RS25990	160407..160718	+	312	WP_152471315.1	hypothetical protein	-
FIV04_RS25995	161110..161946	+	837	WP_152471316.1	hypothetical protein	-
FIV04_RS26000	162141..162473	-	333	WP_152471317.1	hypothetical protein	-
FIV04_RS26005	162647..162807	-	161	Protein_165	ribose ABC transporter permease	-
FIV04_RS26010	163098..163976	+	879	WP_152471318.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..181301	181301

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14804.12 Da
• Isoelectric Point: 7.9088

>T141724 WP_152471335.1 NZ_CP045341:c158837-158436 [Vibrio sp. THAF190c]
MLDTCICSFIMREHPITVLQKLQTVVSNQNRIVISAITYQEMQYGLLGKKASPKHAVLVEEFLKRVDEILPWDKAAVDAT
TNVKRDLMAKGTPIGNNDTAIAGHAISTGSVLVTNNTREFQRVSGLKLEDWVH

Nucleotide

• Length: 402 bp

>T141724 NZ_CP060073:3406321-3406539 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8964.94 Da
• Isoelectric Point: 4.4066

>AT141724 WP_152471312.1 NZ_CP045341:c159084-158851 [Vibrio sp. THAF190c]
MRTVSIFKNGRNQAVRLPKDLEFDGVNELEIHKEGDVITLRPARPDWLSFGNQEKADSDFIVNREDVIEDEGRFNFE

Nucleotide

• Length: 234 bp

>AT141724 NZ_CP060073:3405921-3406295 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG