Detailed information of TA system TA141715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2714004..2714421
• Replicon: chromosome
• Accession: NZ_CP045324
• Organism: Marinobacter sp. THAF197a

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: FIV08_RS12620
• Protein ID: WP_152438564.1
• Coordinates: 2714242..2714421 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: FIV08_RS12615
• Protein ID: WP_152438563.1
• Coordinates: 2714004..2714258 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIV08_RS12590	2710234..2710923	-	690	WP_152438559.1	hypothetical protein	-
FIV08_RS12595	2710960..2711283	-	324	WP_152438560.1	nucleotidyltransferase domain-containing protein	-
FIV08_RS12600	2711642..2713114	+	1473	WP_152438561.1	flagellin	-
FIV08_RS12605	2713318..2713557	+	240	WP_061333890.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FIV08_RS12610	2713559..2713945	+	387	WP_152438562.1	type II toxin-antitoxin system VapC family toxin	-
FIV08_RS12615	2714004..2714258	-	255	WP_152438563.1	BrnA antitoxin family protein	Antitoxin
FIV08_RS12620	2714242..2714421	-	180	WP_152438564.1	BrnT family toxin	Toxin
FIV08_RS12625	2714568..2715968	-	1401	WP_152438565.1	plasmid pRiA4b ORF-3 family protein	-
FIV08_RS12630	2716151..2716384	+	234	WP_152438566.1	DUF2281 domain-containing protein	-
FIV08_RS12640	2716748..2718751	+	2004	WP_152438567.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (3-51)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (24-77)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6815.96 Da
• Isoelectric Point: 7.9630

>T141715 WP_152438564.1 NZ_CP045324:c2714421-2714242 [Marinobacter sp. THAF197a]
MLTAIDDLYAYGEERWIAIGLIKQFIGVVVYVERCGDAVRIISARKAARREVRLYEQNI

Nucleotide

• Length: 180 bp

>T141715 NZ_CP060073:718465-718761 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9925.17 Da
• Isoelectric Point: 7.0135

>AT141715 WP_152438563.1 NZ_CP045324:c2714258-2714004 [Marinobacter sp. THAF197a]
MNRTSKTDWQRMAEHGDEQIDTSDIPELDDAFFERAELRLPAKQPVTLRIDEDVLAWFKAQGKGYQTRINKLLRQYMGSQ
VNRR

Nucleotide

• Length: 255 bp

>AT141715 NZ_CP060073:718763-719158 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCACAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA