Detailed information of TA system TA141698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2147735..2148474
• Replicon: chromosome
• Accession: NZ_CP045312
• Organism: Enterobacter hormaechei strain 14102KSN

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: GC132_RS10225
• Protein ID: WP_038416064.1
• Coordinates: 2147989..2148474 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: GC132_RS10220
• Protein ID: WP_003857131.1
• Coordinates: 2147735..2148001 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GC132_RS10195	2142883..2143407	+	525	WP_087878309.1	fimbrial protein	-
GC132_RS10200	2143431..2144567	+	1137	WP_038416067.1	type 1 fimbrial protein	-
GC132_RS10205	2144688..2145245	+	558	WP_023300131.1	OmpH family outer membrane protein	-
GC132_RS10210	2145224..2146612	-	1389	WP_038416066.1	MFS transporter	-
GC132_RS10215	2146742..2147671	+	930	WP_038416065.1	LysR family transcriptional regulator	-
GC132_RS10220	2147735..2148001	+	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
GC132_RS10225	2147989..2148474	+	486	WP_038416064.1	GNAT family N-acetyltransferase	Toxin
GC132_RS10230	2148918..2150210	-	1293	WP_038416063.1	glycoside hydrolase	-
GC132_RS10235	2150315..2151073	-	759	WP_022648129.1	DeoR/GlpR family DNA-binding transcription regulator	-
GC132_RS10240	2151160..2151744	+	585	WP_022648128.1	NUDIX hydrolase	-
GC132_RS10245	2151829..2152587	+	759	WP_032624253.1	trans-aconitate 2-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17546.28 Da
• Isoelectric Point: 9.9308

>T141698 WP_038416064.1 NZ_CP045312:2147989-2148474 [Enterobacter hormaechei]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALAAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGKGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T141698 NZ_CP060065:2308944-2309231 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT141698 WP_003857131.1 NZ_CP045312:2147735-2148001 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT141698 NZ_CP060065:2308705-2308944 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9