Detailed information of TA system TA141691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK(toxin)
• Location: 50646..51186
• Replicon: plasmid pB1445CHC_150k
• Accession: NZ_CP045305
• Organism: Legionella longbeachae strain B1445CHC

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A7G4RL43
• Locus tag: GCB94_RS00590
• Protein ID: WP_014845117.1
• Coordinates: 50866..51186 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A2H5FRI5
• Locus tag: GCB94_RS00585
• Protein ID: WP_014845118.1
• Coordinates: 50646..50879 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCB94_RS00560	45775..48273	+	2499	WP_014845123.1	DEAD/DEAH box helicase family protein	-
GCB94_RS00565	48453..48734	-	282	WP_014845122.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
GCB94_RS00570	48727..48987	-	261	WP_014845121.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
GCB94_RS00575	49200..50084	+	885	WP_014845120.1	recombinase family protein	-
GCB94_RS00580	50190..50537	+	348	WP_080031944.1	WGR domain-containing protein	-
GCB94_RS00585	50646..50879	+	234	WP_014845118.1	hypothetical protein	Antitoxin
GCB94_RS00590	50866..51186	+	321	WP_014845117.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GCB94_RS00595	51624..52016	-	393	Protein_55	methyltransferase domain-containing protein	-
GCB94_RS00600	52382..52915	-	534	WP_061637559.1	IS91 family transposase	-
GCB94_RS00605	52937..53428	-	492	WP_051009158.1	IS91 family transposase	-
GCB94_RS00610	53500..53655	+	156	WP_154080667.1	hypothetical protein	-
GCB94_RS00615	54020..54607	+	588	WP_014845114.1	Hsp20/alpha crystallin family protein	-
GCB94_RS00620	54755..55246	+	492	WP_014845113.1	Hsp20/alpha crystallin family protein	-
GCB94_RS00625	55872..56174	+	303	WP_014845112.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-29	-	1..150426	150426

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11444.32 Da
• Isoelectric Point: 9.4321

>T141691 WP_014845117.1 NZ_CP045305:50866-51186 [Legionella longbeachae]
MNRGDVYWVNLDPTTGSEINKLRPCVLVGATPINQARRTVVVVPLSSSATARPPITITVSCLGKQVTAVCDQIRTIDKSR
LKKVAGNLSDKDLNALDDGLRQVLCL

Nucleotide

• Length: 321 bp

>T141691 NZ_CP060065:c656416-655952 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8932.14 Da
• Isoelectric Point: 4.8047

>AT141691 WP_014845118.1 NZ_CP045305:50646-50879 [Legionella longbeachae]
MNKVLISIPDQIASRMRAAIPQRQRSKVIAHLIEKEIERREKALYECALAVENDHGLQNEMNDWDITVQDGLTDESW

Nucleotide

• Length: 234 bp

>AT141691 NZ_CP060065:c656751-656416 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A7G4RL43

Antitoxin

• AlphaFold DB: A0A2H5FRI5