Detailed information of TA system TA141689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 303599..304028
• Replicon: plasmid unnamed1
• Accession: NZ_CP045303
• Organism: Azotobacter salinestris strain KACC 13899

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GCU53_RS25245
• Protein ID: WP_152390264.1
• Coordinates: 303599..303799 (-)
• Length: 67 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GCU53_RS25250
• Protein ID: WP_090624652.1
• Coordinates: 303789..304028 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU53_RS25210	298970..299515	-	546	WP_152390050.1	hypothetical protein	-
GCU53_RS25215	299521..299709	-	189	Protein_328	virulence RhuM family protein	-
GCU53_RS25220	299801..299965	-	165	Protein_329	virulence RhuM family protein	-
GCU53_RS25225	300513..301868	+	1356	WP_167520130.1	ATP-binding protein	-
GCU53_RS25235	302334..302429	-	96	Protein_331	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
GCU53_RS25240	302447..303508	-	1062	WP_152390263.1	transposase	-
GCU53_RS25245	303599..303799	-	201	WP_152390264.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GCU53_RS25250	303789..304028	-	240	WP_090624652.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GCU53_RS25255	304586..305008	+	423	WP_152390265.1	DoxX family protein	-
GCU53_RS25260	305057..305785	+	729	WP_152390266.1	pirin family protein	-
GCU53_RS25265	305892..306848	+	957	WP_152390267.1	zinc-binding alcohol dehydrogenase family protein	-
GCU53_RS25270	307521..307784	+	264	WP_152390268.1	hypothetical protein	-
GCU53_RS25275	307863..308468	+	606	WP_152390100.1	3'-5' exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..321600	321600

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-65)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 67 a.a.
• Molecular weight: 8002.30 Da
• Isoelectric Point: 10.0643

>T141689 WP_152390264.1 NZ_CP045303:c303799-303599 [Azotobacter salinestris]
MTYSLEFDARALKEWHKLGDTVRQQFKKKLIEVLEHPRIEANRLHSLPDCYKIKLRGNGYRLVYRE

Nucleotide

• Length: 201 bp

>T141689 NZ_CP060062:c36590-36204 [Escherichia coli]
ATGAAATTCGTTAGTTCACAGGAAGTGATTGAGTTTCATGACCGCTTGATTTCGCGTGATGGTGGCGTGGCGGGTATGCC
AGAACCCGGTCGCGCTGACGCGATCATTCATCGTGTTTTGAACATGTATCATTATGAAGGTGTGACAGATATTATGGATC
TGGCTGCCGTGTATTTAGTTGCTATTGCTCGTGGTCACATTTTCAACGATGCAAATAAGCGAACAGCCTTGTTTGTTGCA
CAGGTTTTTTTAAAACGGAATGGTGTACATATTATTAGTTCACGTATTTCGTTTGATGAAATGCAAATTATCGCTTTGAA
TGCTGCAACAGGAGAATATAACTGGAAGATGGTTTCTGACCATCTTAAAGCCATTATTCTAAATTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9140.51 Da
• Isoelectric Point: 4.6437

>AT141689 WP_090624652.1 NZ_CP045303:c304028-303789 [Azotobacter salinestris]
MASINIRIDDDLKDRAYRELERLGVTPSELMRQTLQYVAERGQLPFRPVLMTEEEEALIATVRERLAAPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT141689 NZ_CP060062:c36811-36587 [Escherichia coli]
ATGTTTACATGTAACTATAGCCATGCTCGCGATAACCTGGCCTCGTTGCTCGAGCTGGCGGTTGATGAAAATCAGCCAGT
TGAGATTCAGCGCCGTAAAGGTGGCGCAGCTGTACTGGTTGATAAAGACGCTTTTGAACGATACCGTAAAGCGGCTCTTG
ATGCTGAGTTTGCTGAGATCATGCAAAGTCATGGGACGGCCTATACGGAGCTTTCTAAGCGATGA