Detailed information of TA system TA141688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 294817..295774
• Replicon: plasmid unnamed1
• Accession: NZ_CP045303
• Organism: Azotobacter salinestris strain KACC 13899

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: GCU53_RS25190
• Protein ID: WP_152390293.1
• Coordinates: 295313..295774 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: GCU53_RS25185
• Protein ID: WP_152390261.1
• Coordinates: 294817..295272 (+)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU53_RS25160	290031..291065	+	1035	WP_152390259.1	IS630 family transposase	-
GCU53_RS25165	291121..291864	+	744	WP_208845548.1	HipA domain-containing protein	-
GCU53_RS25170	291940..293019	+	1080	WP_208845541.1	IS630 family transposase	-
GCU53_RS25175	293285..293776	+	492	Protein_321	tyrosine-type recombinase/integrase	-
GCU53_RS25180	293863..294621	-	759	WP_152386438.1	IS5 family transposase	-
GCU53_RS25185	294817..295272	+	456	WP_152390261.1	helix-turn-helix domain-containing protein	Antitoxin
GCU53_RS25190	295313..295774	+	462	WP_152390293.1	PIN domain-containing protein	Toxin
GCU53_RS25195	296144..296317	+	174	Protein_325	site-specific integrase	-
GCU53_RS25205	296608..298977	-	2370	WP_152390051.1	DEAD/DEAH box helicase family protein	-
GCU53_RS25210	298970..299515	-	546	WP_152390050.1	hypothetical protein	-
GCU53_RS25215	299521..299709	-	189	Protein_328	virulence RhuM family protein	-
GCU53_RS25220	299801..299965	-	165	Protein_329	virulence RhuM family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..321600	321600

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-101)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 17260.72 Da
• Isoelectric Point: 6.2233

>T141688 WP_152390293.1 NZ_CP045303:295313-295774 [Azotobacter salinestris]
MYPAPLRSLLMYLALSGQFRARWSETIHQEWTRNLLADRPDLSPDTVGRVRELMDRHVPGALVTGFESLIPGITLPDPED
RHVVAAAVQTRAEAIVTFNLKDFPDQDLSPFGVRAIHPDDFITDLMELHIGAVLEAVRRHRASLKNPPFSSRE

Nucleotide

• Length: 462 bp

>T141688 NZ_CP060062:28178-28459 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACCGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGAATTGGTTATTCAGCCAGCAGCCTGGCTGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 16573.01 Da
• Isoelectric Point: 5.0439

>AT141688 WP_152390261.1 NZ_CP045303:294817-295272 [Azotobacter salinestris]
MNRLQETLPSAAEAALAKLCSQELSAIIETRAETQTLSISDPKGGRSVNIPVSALRLLVDVLTELGEGNSVRLVPVHAEL
TTQEAADLLNMSRPTLIKLLDEEAIPYHRAGNRRKVKFADLRIYQEKLEQERLAALAELSALDRALGLGYE

Nucleotide

• Length: 456 bp

>AT141688 NZ_CP060062:27912-28181 [Escherichia coli]
ATGACACAGGTTAACATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGCGGCGATGTGCTTTCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA