Detailed information of TA system TA141686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 259542..260166
• Replicon: plasmid unnamed1
• Accession: NZ_CP045303
• Organism: Azotobacter salinestris strain KACC 13899

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GCU53_RS25000
• Protein ID: WP_152390291.1
• Coordinates: 259542..259730 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0C4WXU9
• Locus tag: GCU53_RS25005
• Protein ID: WP_040107336.1
• Coordinates: 259762..260166 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU53_RS24970	254843..255556	-	714	WP_152390237.1	type 1 glutamine amidotransferase domain-containing protein	-
GCU53_RS24975	255655..256590	+	936	WP_091014019.1	LysR family transcriptional regulator	-
GCU53_RS24980	256626..256724	-	99	WP_089170148.1	IS66 family insertion sequence hypothetical protein	-
GCU53_RS24985	256760..257083	-	324	WP_152390238.1	transposase	-
GCU53_RS24990	257463..258701	-	1239	WP_152390239.1	IS110 family transposase	-
GCU53_RS25000	259542..259730	+	189	WP_152390291.1	type II toxin-antitoxin system HicA family toxin	Toxin
GCU53_RS25005	259762..260166	+	405	WP_040107336.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GCU53_RS25015	260669..261707	-	1039	Protein_292	IS630 family transposase	-
GCU53_RS25020	261934..262542	+	609	WP_152390240.1	cupin	-
GCU53_RS25025	262632..263906	-	1275	WP_152390241.1	OprD family porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..321600	321600
	-	flank	IS/Tn	-	-	256626..256763	137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-61)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7035.11 Da
• Isoelectric Point: 10.4803

>T141686 WP_152390291.1 NZ_CP045303:259542-259730 [Azotobacter salinestris]
MRSREVIELLEADGWYEVAVKGSHHQFKHPTKPGRVTVPHPHPKSDLPKGTWQSILKQAGLK

Nucleotide

• Length: 189 bp

>T141686 NZ_CP060061:4482669-4482926 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14695.64 Da
• Isoelectric Point: 4.6292

>AT141686 WP_040107336.1 NZ_CP045303:259762-260166 [Azotobacter salinestris]
MKYPVVIHKEQSSDYGVTVPDVPGCFSAGETMTEALENVREALELHFEGLLADGEPLPQPQEIDVHAANPDYAGGVWALV
DFDVMPYLGKAVRFNATLPEHLLARIDAYVKAHPEQKSRSGFLAEAALRVLQHA

Nucleotide

• Length: 405 bp

>AT141686 NZ_CP060061:4482938-4483483 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTTAGCAAAGAAGCCGATTTAGTGACCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0C4WXU9