Detailed information of TA system TA141684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/Phd(antitoxin)
• Location: 126593..127191
• Replicon: plasmid unnamed1
• Accession: NZ_CP045303
• Organism: Azotobacter salinestris strain KACC 13899

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: GCU53_RS24335
• Protein ID: WP_157731523.1
• Coordinates: 126850..127191 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0C4WLQ2
• Locus tag: GCU53_RS24330
• Protein ID: WP_040107296.1
• Coordinates: 126593..126853 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU53_RS24305	122685..123161	+	477	WP_152390138.1	single-stranded DNA-binding protein	-
GCU53_RS24310	123313..124464	+	1152	WP_152390139.1	recombinase RecT	-
GCU53_RS24315	124601..124780	+	180	WP_090732744.1	hypothetical protein	-
GCU53_RS24320	124989..125462	+	474	WP_152390140.1	hypothetical protein	-
GCU53_RS24325	125449..126489	+	1041	WP_152390141.1	YqaJ viral recombinase family protein	-
GCU53_RS24330	126593..126853	+	261	WP_040107296.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GCU53_RS24335	126850..127191	+	342	WP_157731523.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GCU53_RS24340	127354..127653	+	300	WP_152390143.1	hypothetical protein	-
GCU53_RS24345	127890..130493	+	2604	WP_152390144.1	TraI domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..321600	321600

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-99)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13012.83 Da
• Isoelectric Point: 5.1133

>T141684 WP_157731523.1 NZ_CP045303:126850-127191 [Azotobacter salinestris]
MSFDVQFATTAETSLFSQIAHLEPYHGYDGAYEKLSTLVAEVIRRLADHPLGYPISAQASELGITHYRELNLDGYRVFYE
CDEEKRLVVVLLILGQRQSIEQQLIHYCLMFAR

Nucleotide

• Length: 342 bp

>T141684 NZ_CP060061:4089949-4090227 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9567.10 Da
• Isoelectric Point: 9.0005

>AT141684 WP_040107296.1 NZ_CP045303:126593-126853 [Azotobacter salinestris]
MKIETISFLKKHAASLDLTEPMVITQNGLPAYVIESYAERKQRDETIALVKLLALGSRDKEQGKLVSGDTLLDRLAQRCQ
QPKGTP

Nucleotide

• Length: 261 bp

>AT141684 NZ_CP060061:4089686-4089946 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACACGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0C4WLQ2