Detailed information of TA system TA141682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 73427..74064
• Replicon: plasmid unnamed1
• Accession: NZ_CP045303
• Organism: Azotobacter salinestris strain KACC 13899

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: GCU53_RS23990
• Protein ID: WP_152390090.1
• Coordinates: 73654..74064 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: GCU53_RS23985
• Protein ID: WP_152390089.1
• Coordinates: 73427..73657 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU53_RS23950	68617..68784	-	168	WP_167520123.1	hypothetical protein	-
GCU53_RS23955	68885..69247	+	363	Protein_86	helix-turn-helix domain containing protein	-
GCU53_RS23960	69253..69855	-	603	Protein_87	SDR family NAD(P)-dependent oxidoreductase	-
GCU53_RS23965	69876..70880	-	1005	WP_152390279.1	zinc-binding dehydrogenase	-
GCU53_RS23970	70900..71853	-	954	WP_152390088.1	cyclase family protein	-
GCU53_RS23975	72355..72804	+	450	WP_152390068.1	DUF2384 domain-containing protein	-
GCU53_RS23980	72801..73238	+	438	WP_152390067.1	RES family NAD+ phosphorylase	-
GCU53_RS23985	73427..73657	+	231	WP_152390089.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GCU53_RS23990	73654..74064	+	411	WP_152390090.1	type II toxin-antitoxin system VapC family toxin	Toxin
GCU53_RS23995	74095..74956	+	862	WP_152390091.1	IS5 family transposase	-
GCU53_RS24000	74943..76199	-	1257	WP_152390092.1	IS701 family transposase	-
GCU53_RS24005	76286..76717	-	432	WP_152390093.1	IS200/IS605 family transposase	-
GCU53_RS24010	76824..77156	-	333	WP_152390094.1	DUF2790 domain-containing protein	-
GCU53_RS25810	77655..77993	-	339	WP_208845542.1	hypothetical protein	-
GCU53_RS25815	77993..78316	-	324	WP_208845543.1	hypothetical protein	-
GCU53_RS24020	78313..78735	-	423	WP_152390095.1	type II toxin-antitoxin system VapC family toxin	-
GCU53_RS24025	78735..78986	-	252	WP_152390096.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..321600	321600

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14938.23 Da
• Isoelectric Point: 5.7324

>T141682 WP_152390090.1 NZ_CP045303:73654-74064 [Azotobacter salinestris]
VTTYMLDTCICSFIMRELPESVLQRLAAEVGQNNRIVISAVTYAEMRFGQIGKKASAKHKTLVDEFVKRLDAVLPWDQRA
VDATVEVMRALTEVGTPIGPNDTAIAGHAIASGCTLVTNNVREFGRVPGLVYEDWV

Nucleotide

• Length: 411 bp

>T141682 NZ_CP060061:3848982-3849200 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8597.83 Da
• Isoelectric Point: 4.7405

>AT141682 WP_152390089.1 NZ_CP045303:73427-73657 [Azotobacter salinestris]
MRTVSIFKNGKNQAIRLPNDMAYEGIGELEIMREGDVITLRPVRPTWQSLAEVPKADADFLQERPPVVGDEGRFNL

Nucleotide

• Length: 231 bp

>AT141682 NZ_CP060061:3848582-3848956 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG