Detailed information of TA system TA141680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 62780..63573
• Replicon: plasmid unnamed1
• Accession: NZ_CP045303
• Organism: Azotobacter salinestris strain KACC 13899

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: GCU53_RS23920
• Protein ID: WP_152390081.1
• Coordinates: 62780..63298 (-)
• Length: 173 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: GCU53_RS23925
• Protein ID: WP_152390082.1
• Coordinates: 63298..63573 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU53_RS23900	58297..58770	+	474	WP_152390078.1	hypothetical protein	-
GCU53_RS23905	59064..61304	+	2241	Protein_76	type VI secretion system protein ImpL	-
GCU53_RS23910	61321..62421	+	1101	WP_152390079.1	type VI secretion system protein TssA	-
GCU53_RS23915	62482..62763	+	282	WP_152390080.1	hypothetical protein	-
GCU53_RS23920	62780..63298	-	519	WP_152390081.1	GNAT family N-acetyltransferase	Toxin
GCU53_RS23925	63298..63573	-	276	WP_152390082.1	DUF1778 domain-containing protein	Antitoxin
GCU53_RS23930	63856..64749	-	894	WP_152390083.1	LysR family transcriptional regulator	-
GCU53_RS23935	64862..66043	+	1182	WP_152390084.1	serine hydrolase	-
GCU53_RS23940	66127..67311	+	1185	WP_152390085.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..321600	321600

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-91)

Sequences

Toxin

Protein

• Length: 173 a.a.
• Molecular weight: 18695.54 Da
• Isoelectric Point: 9.4747

>T141680 WP_152390081.1 NZ_CP045303:c63298-62780 [Azotobacter salinestris]
MSLTAPQALDASHSLEGFDCGKPSLDDWLMRHARQAQASGSAKTYVVADGVRVAGYYSLTVGQIDTLEAPARVRQGMGAY
PIPVVILARMAVTRQDQGRGIGVGMLQDAIRRTLTIAEQAGVRALLTHPIDEEALRFYLRFGFEASPAREQQLLLLLKDA
RRLLVPPASPRG

Nucleotide

• Length: 519 bp

>T141680 NZ_CP045303:c63298-62780 [Azotobacter salinestris]
ATGTCGCTGACCGCGCCCCAAGCACTGGACGCCAGCCACTCACTGGAGGGTTTCGATTGCGGCAAACCGAGCCTGGATGA
TTGGCTGATGCGCCATGCCCGGCAGGCGCAGGCCAGCGGTTCGGCCAAGACCTACGTAGTCGCTGACGGCGTGCGCGTCG
CGGGCTACTACAGCCTCACCGTGGGACAGATCGATACCCTGGAAGCTCCCGCTCGTGTCCGCCAGGGCATGGGGGCTTAT
CCCATACCTGTTGTCATCCTGGCGCGGATGGCCGTGACGCGACAGGATCAAGGGCGCGGCATCGGCGTAGGCATGCTGCA
GGATGCGATCCGGCGTACCCTCACCATCGCCGAGCAGGCCGGCGTTCGGGCGCTGTTGACGCATCCGATCGACGAGGAGG
CATTACGCTTCTACCTGCGCTTCGGCTTCGAAGCATCGCCTGCCCGCGAGCAGCAATTGCTGTTGCTGCTCAAGGACGCG
CGTCGGCTGCTTGTTCCGCCGGCGTCCCCCAGGGGATAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10008.35 Da
• Isoelectric Point: 6.2594

>AT141680 WP_152390082.1 NZ_CP045303:c63573-63298 [Azotobacter salinestris]
MASTIRSARLGLRATPQQEAVLRRAAEVANKSMTDFILDSACQAAEQTLLDQRLFLVSGSQSQALLEMLDRPAQDNSGLK
DLFSRSAPWDK

Nucleotide

• Length: 276 bp

>AT141680 NZ_CP045303:c63573-63298 [Azotobacter salinestris]
ATGGCAAGTACCATCCGTTCTGCCCGTCTTGGGTTGCGCGCCACGCCGCAGCAGGAAGCGGTGCTGCGCCGCGCGGCCGA
GGTGGCAAACAAATCGATGACCGATTTTATCCTCGACAGTGCCTGTCAGGCCGCCGAGCAGACCTTGCTCGATCAACGCC
TGTTTCTGGTATCCGGCAGCCAGTCCCAAGCCTTGCTGGAGATGCTGGATCGGCCTGCCCAAGACAATTCTGGCCTGAAG
GATTTGTTCTCGCGATCGGCACCTTGGGACAAATGA