Detailed information of TA system TA141679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 34965..35848
• Replicon: plasmid unnamed1
• Accession: NZ_CP045303
• Organism: Azotobacter salinestris strain KACC 13899

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GCU53_RS23775
• Protein ID: WP_152390067.1
• Coordinates: 34965..35402 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: PrcA
• Uniprot ID: -
• Locus tag: GCU53_RS23780
• Protein ID: WP_152390068.1
• Coordinates: 35399..35848 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU53_RS23730	30638..31453	-	816	Protein_40	transposase	-
GCU53_RS23735	31456..31725	-	270	WP_152390063.1	type II toxin-antitoxin system YafQ family toxin	-
GCU53_RS23740	31712..31972	-	261	WP_152390064.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
GCU53_RS23745	31987..32259	-	273	WP_152390065.1	hypothetical protein	-
GCU53_RS23750	32274..32621	-	348	WP_131300956.1	type II toxin-antitoxin system ChpB family toxin	-
GCU53_RS23755	32618..32872	-	255	WP_131300954.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
GCU53_RS23760	32946..33272	-	327	WP_152390277.1	integrase	-
GCU53_RS23765	33489..34550	+	1062	WP_152390066.1	transposase	-
GCU53_RS23770	34562..34687	-	126	Protein_48	integrase	-
GCU53_RS23775	34965..35402	-	438	WP_152390067.1	RES family NAD+ phosphorylase	Toxin
GCU53_RS23780	35399..35848	-	450	WP_152390068.1	DUF2384 domain-containing protein	Antitoxin
GCU53_RS23785	36065..36553	+	489	WP_152390069.1	hypothetical protein	-
GCU53_RS23790	36759..38372	-	1614	WP_152390070.1	ATP-binding cassette domain-containing protein	-
GCU53_RS23795	39001..39360	-	360	WP_136888326.1	transcriptional regulator	-
GCU53_RS23800	39360..39662	-	303	WP_136888327.1	type II toxin-antitoxin system HigB family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..321600	321600

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-140)

Antitoxin

• Antitoxin Xre (23-147)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15876.30 Da
• Isoelectric Point: 5.5399

>T141679 WP_152390067.1 NZ_CP045303:c35402-34965 [Azotobacter salinestris]
VILWRISAYADLSGVGGLRASGRWHHLGRPVVYAADSPAGAMLEVLVHLEIDPEDFPTTLQLIRIELPDPVSRAELPPLP
EHWKSAPEVTRAMGDRFLDGRSALLLPVPSAIIPCTTNYLFNPVHPEASGASIQVEKFPLDSRLI

Nucleotide

• Length: 438 bp

>T141679 NZ_CP060061:c2787860-2787606 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16938.48 Da
• Isoelectric Point: 6.7345

>AT141679 WP_152390068.1 NZ_CP045303:c35848-35399 [Azotobacter salinestris]
MLAEVLRDHGYTAYRARLQSLLDIPESATDFEIHTRITKGFPATRLVQLTEAGVVTPLERDQIIPLRTLKSRIERDQPLT
VEESDRFFRAAHITAMAEAVFGDREKAKRWLSKPKERFSGLTPMQMLTTQQGTSQVEEMLLQIAEGLAL

Nucleotide

• Length: 450 bp

>AT141679 NZ_CP060061:c2788108-2787857 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA