Detailed information of TA system TA141678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-MazE
• Location: 32274..32872
• Replicon: plasmid unnamed1
• Accession: NZ_CP045303
• Organism: Azotobacter salinestris strain KACC 13899

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: GCU53_RS23750
• Protein ID: WP_131300956.1
• Coordinates: 32274..32621 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: -
• Locus tag: GCU53_RS23755
• Protein ID: WP_131300954.1
• Coordinates: 32618..32872 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU53_RS23715	28014..28748	-	735	WP_152390061.1	two-component system response regulator OmpR	-
GCU53_RS25610	28954..29100	+	147	WP_167520121.1	hypothetical protein	-
GCU53_RS23720	29173..29427	+	255	WP_152390276.1	EF-hand domain-containing protein	-
GCU53_RS23725	29440..30288	+	849	WP_152390062.1	sterol desaturase family protein	-
GCU53_RS23730	30638..31453	-	816	Protein_40	transposase	-
GCU53_RS23735	31456..31725	-	270	WP_152390063.1	type II toxin-antitoxin system YafQ family toxin	-
GCU53_RS23740	31712..31972	-	261	WP_152390064.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
GCU53_RS23745	31987..32259	-	273	WP_152390065.1	hypothetical protein	-
GCU53_RS23750	32274..32621	-	348	WP_131300956.1	type II toxin-antitoxin system ChpB family toxin	Toxin
GCU53_RS23755	32618..32872	-	255	WP_131300954.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GCU53_RS23760	32946..33272	-	327	WP_152390277.1	integrase	-
GCU53_RS23765	33489..34550	+	1062	WP_152390066.1	transposase	-
GCU53_RS23770	34562..34687	-	126	Protein_48	integrase	-
GCU53_RS23775	34965..35402	-	438	WP_152390067.1	RES family NAD+ phosphorylase	-
GCU53_RS23780	35399..35848	-	450	WP_152390068.1	DUF2384 domain-containing protein	-
GCU53_RS23785	36065..36553	+	489	WP_152390069.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..321600	321600

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-114)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12227.87 Da
• Isoelectric Point: 8.5410

>T141678 WP_131300956.1 NZ_CP045303:c32621-32274 [Azotobacter salinestris]
VRRAKYGRGDIVRLNLNPTAGREQQGDFRPALILTPAAYNATGLAVIAPITQGGDHARYAGFAVTLSGAGTETQGVILTN
QIRSVDLEARGAKRVESVPEVVIEDALARVQALFE

Nucleotide

• Length: 348 bp

>T141678 NZ_CP060061:2775964-2776338 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9047.50 Da
• Isoelectric Point: 6.2345

>AT141678 WP_131300954.1 NZ_CP045303:c32872-32618 [Azotobacter salinestris]
MEVKIQQWGNSAAIRLPATVLKQMSLASGDVLSLDVTGEAITLKPARAKPRYKLADLMAQCDLNAPEPAELAAWNSMQPV
GREA

Nucleotide

• Length: 255 bp

>AT141678 NZ_CP060061:2775507-2775875 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA