Detailed information of TA system TA141677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 31456..31972
• Replicon: plasmid unnamed1
• Accession: NZ_CP045303
• Organism: Azotobacter salinestris strain KACC 13899

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GCU53_RS23735
• Protein ID: WP_152390063.1
• Coordinates: 31456..31725 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GCU53_RS23740
• Protein ID: WP_152390064.1
• Coordinates: 31712..31972 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU53_RS23710	26713..28017	-	1305	WP_152390060.1	HAMP domain-containing protein	-
GCU53_RS23715	28014..28748	-	735	WP_152390061.1	two-component system response regulator OmpR	-
GCU53_RS25610	28954..29100	+	147	WP_167520121.1	hypothetical protein	-
GCU53_RS23720	29173..29427	+	255	WP_152390276.1	EF-hand domain-containing protein	-
GCU53_RS23725	29440..30288	+	849	WP_152390062.1	sterol desaturase family protein	-
GCU53_RS23730	30638..31453	-	816	Protein_40	transposase	-
GCU53_RS23735	31456..31725	-	270	WP_152390063.1	type II toxin-antitoxin system YafQ family toxin	Toxin
GCU53_RS23740	31712..31972	-	261	WP_152390064.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GCU53_RS23745	31987..32259	-	273	WP_152390065.1	hypothetical protein	-
GCU53_RS23750	32274..32621	-	348	WP_131300956.1	type II toxin-antitoxin system ChpB family toxin	-
GCU53_RS23755	32618..32872	-	255	WP_131300954.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
GCU53_RS23760	32946..33272	-	327	WP_152390277.1	integrase	-
GCU53_RS23765	33489..34550	+	1062	WP_152390066.1	transposase	-
GCU53_RS23770	34562..34687	-	126	Protein_48	integrase	-
GCU53_RS23775	34965..35402	-	438	WP_152390067.1	RES family NAD+ phosphorylase	-
GCU53_RS23780	35399..35848	-	450	WP_152390068.1	DUF2384 domain-containing protein	-
GCU53_RS23785	36065..36553	+	489	WP_152390069.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..321600	321600

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-87)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (6-83)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10234.81 Da
• Isoelectric Point: 9.0873

>T141677 WP_152390063.1 NZ_CP045303:c31725-31456 [Azotobacter salinestris]
VRTIKQTGQFKRDLKREVKGPLEQDLLPVIEVLAKDRPLEPKHRDHALSGNWADHRDCHIKPDLVLLYRKPGDETLQLVR
LGSHSELGL

Nucleotide

• Length: 270 bp

>T141677 NZ_CP060061:c2504792-2504689 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9476.86 Da
• Isoelectric Point: 4.4400

>AT141677 WP_152390064.1 NZ_CP045303:c31972-31712 [Azotobacter salinestris]
MATETTVRARVDERIKHEATIVLESMGLTMSDAIKLMLVRVAEEGRLPFAPLEPSLETIAAAREAREGNLETVTLDELRA
AIRADD

Nucleotide

• Length: 261 bp

>AT141677 NZ_CP060061:2504651-2504922 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT