Detailed information of TA system TA141676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 8463..8940
• Replicon: plasmid unnamed1
• Accession: NZ_CP045303
• Organism: Azotobacter salinestris strain KACC 13899

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: GCU53_RS23580
• Protein ID: WP_152390043.1
• Coordinates: 8674..8940 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: GCU53_RS23575
• Protein ID: WP_152390042.1
• Coordinates: 8463..8690 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU53_RS25605	3770..3931	-	162	WP_167520120.1	hypothetical protein	-
GCU53_RS23550	4354..4689	+	336	WP_152390040.1	hypothetical protein	-
GCU53_RS23555	5005..5244	+	240	WP_152390275.1	hypothetical protein	-
GCU53_RS23560	5294..5590	+	297	WP_152390041.1	HU family DNA-binding protein	-
GCU53_RS23565	5959..6994	+	1036	Protein_8	IS630 family transposase	-
GCU53_RS23570	7058..8276	+	1219	Protein_9	ATP-binding protein	-
GCU53_RS23575	8463..8690	+	228	WP_152390042.1	CopG family transcriptional regulator	Antitoxin
GCU53_RS23580	8674..8940	+	267	WP_152390043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GCU53_RS23585	9757..10047	+	291	WP_152390044.1	nucleotidyltransferase family protein	-
GCU53_RS23590	10640..11236	-	597	WP_152390045.1	putative adenosine monophosphate-protein transferase Fic	-
GCU53_RS23595	11239..11406	-	168	WP_089170224.1	YhfG family protein	-
GCU53_RS23600	11604..12560	-	957	WP_152390046.1	zinc-binding alcohol dehydrogenase family protein	-
GCU53_RS23605	12660..13375	-	716	Protein_16	pirin family protein	-
GCU53_RS23610	13424..13846	-	423	WP_152390047.1	DoxX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..321600	321600
	-	flank	IS/Tn	-	-	6407..6994	587

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10180.75 Da
• Isoelectric Point: 8.9999

>T141676 WP_152390043.1 NZ_CP045303:8674-8940 [Azotobacter salinestris]
MAWTIDYTQTALNQLRKLDKQSARLILDFMDEQVALREDPRSTGKALTGPLGGLWRYRVGDFRVICEIQDGALRVLVLEL
GNRREVYR

Nucleotide

• Length: 267 bp

>T141676 NZ_CP060061:2211588-2211917 [Escherichia coli]
ATGAACTACATTATCGAATACTACAGTGACGAGGTTGAAGCTGAGATCCTTTCTCTTCCTGAGACCTTACAAGCCCGGTA
CATTCGGTACACGGAGAAAATGCGTATTTACGGAGCTAATCTTGGTTCCCCGCATACAGAGGCATTTGGCGACGGTTTGT
TTGAAATCCGCCTTAAGGGCTCGGAAGGCATAGGGCGTGTTTTTTACTGCACGCTAAAAGGAAAGCGAATCATTATGTTG
CATAGCTTTGTAAAGAAAACGCAAAAAACTCCACCTGCCGAGCTTAGAAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8669.75 Da
• Isoelectric Point: 4.5098

>AT141676 WP_152390042.1 NZ_CP045303:8463-8690 [Azotobacter salinestris]
MPTSIRLAPEIEQRLDFLAANTGRTKAYYLREIIERGLDEMEDYYLAAEVLERVRKGQEQVHSSADVRKDLGLDD

Nucleotide

• Length: 228 bp

>AT141676 NZ_CP060061:2211907-2212215 [Escherichia coli]
ATGACTGGTAAACGTACCCCACCTACCATGACACACGATGATATGGTAGAAAAAATGTTGTCTAACCCCGCAGTAAAAGC
AGAATACGACGCCATCGCCGATGAATTCGCACTACTTGATGAAATGCTGGCAGCACGAAAAGAAGCTGGCTTAACCCAGG
CTGAAGTTGCTGAACGTATGGGAACAAAAGCGACTGCGATAACCAGAATGGAAAGCAATCTTGCATCAGGTACAAGCGGC
CCATCATTTGCCACACTGAAAAAATTCGCCCGTGCTACAGGGAAAAAACTCCAGATCCGCTTCGTTTAA