Detailed information of TA system TA141675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4802739..4803303
• Replicon: chromosome
• Accession: NZ_CP045302
• Organism: Azotobacter salinestris strain KACC 13899

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GCU53_RS22765
• Protein ID: WP_152389610.1
• Coordinates: 4803016..4803303 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GCU53_RS22760
• Protein ID: WP_152389609.1
• Coordinates: 4802739..4803026 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU53_RS22730	4797898..4799409	-	1512	WP_152389606.1	IS66 family transposase	-
GCU53_RS25595	4799491..4799826	-	336	WP_167520046.1	IS66 family insertion sequence element accessory protein TnpB	-
GCU53_RS22740	4799823..4800152	-	330	WP_152388408.1	transposase	-
GCU53_RS22745	4801144..4801359	-	216	WP_152389607.1	hypothetical protein	-
GCU53_RS22750	4801952..4802266	+	315	WP_152389608.1	DUF1654 domain-containing protein	-
GCU53_RS22755	4802374..4802650	+	277	Protein_4371	hypothetical protein	-
GCU53_RS22760	4802739..4803026	+	288	WP_152389609.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GCU53_RS22765	4803016..4803303	+	288	WP_152389610.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GCU53_RS22770	4803358..4803810	+	453	WP_152389611.1	DUF2442 domain-containing protein	-
GCU53_RS22775	4803883..4804134	-	252	WP_152389612.1	DUF1654 domain-containing protein	-
GCU53_RS22780	4804286..4804543	+	258	WP_152389613.1	hypothetical protein	-
GCU53_RS22785	4804667..4804903	+	237	WP_152389614.1	hypothetical protein	-
GCU53_RS22790	4805000..4806073	-	1074	WP_152389615.1	ImmA/IrrE family metallo-endopeptidase	-
GCU53_RS22795	4806060..4806698	-	639	WP_152389616.1	hypothetical protein	-
GCU53_RS22800	4806894..4807766	-	873	WP_152390025.1	XRE family transcriptional regulator	-
GCU53_RS22805	4807854..4808042	+	189	WP_152389617.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4792137..4840805	48668

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-71)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11006.98 Da
• Isoelectric Point: 10.6762

>T141675 WP_152389610.1 NZ_CP045302:4803016-4803303 [Azotobacter salinestris]
MTVEYAVEWAPQALKELRKLGGAIRLQLLRKLDERRRNPRVPGDALHGLKDCYKIKLKSAGYRLVYRVEDERVVVVVVAV
GKRERSAVYEQAMKR

Nucleotide

• Length: 288 bp

>T141675 NZ_CP060061:c2142915-2142628 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10443.89 Da
• Isoelectric Point: 4.3423

>AT141675 WP_152389609.1 NZ_CP045302:4802739-4803026 [Azotobacter salinestris]
MQSILADVAVSVSELKKNPSAVLSSAGGAPVAVLNHNRVMGYMVPADVFEAMMERLDDLELIRVARERIEANEPTVRVAL
EDLIAEAERDTEHDR

Nucleotide

• Length: 288 bp

>AT141675 NZ_CP060061:c2143154-2142915 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA