Detailed information of TA system TA141673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 4121046..4121648
• Replicon: chromosome
• Accession: NZ_CP045302
• Organism: Azotobacter salinestris strain KACC 13899

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: GCU53_RS19310
• Protein ID: WP_152389045.1
• Coordinates: 4121358..4121648 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GCU53_RS19305
• Protein ID: WP_152389044.1
• Coordinates: 4121046..4121348 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU53_RS19265	4116407..4116850	-	444	WP_152389039.1	type II 3-dehydroquinate dehydratase	-
GCU53_RS19275	4117421..4118707	+	1287	WP_152389040.1	integrase arm-type DNA-binding domain-containing protein	-
GCU53_RS25800	4118901..4119608	+	708	WP_208845326.1	hypothetical protein	-
GCU53_RS19285	4119836..4120018	+	183	WP_152389967.1	transcriptional regulator	-
GCU53_RS19290	4120019..4120327	+	309	WP_152389041.1	hypothetical protein	-
GCU53_RS19295	4120324..4120563	+	240	WP_152389042.1	hypothetical protein	-
GCU53_RS19300	4120784..4121035	-	252	WP_152389043.1	BrnA antitoxin family protein	-
GCU53_RS19305	4121046..4121348	-	303	WP_152389044.1	putative addiction module antidote protein	Antitoxin
GCU53_RS19310	4121358..4121648	-	291	WP_152389045.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GCU53_RS25565	4121974..4122144	+	171	WP_167520088.1	hypothetical protein	-
GCU53_RS19320	4122141..4122443	+	303	WP_152389047.1	hypothetical protein	-
GCU53_RS19325	4122809..4123348	+	540	WP_152389048.1	hypothetical protein	-
GCU53_RS19330	4123345..4123566	+	222	WP_152389049.1	hypothetical protein	-
GCU53_RS19335	4123563..4125677	+	2115	WP_152389050.1	DUF927 domain-containing protein	-
GCU53_RS19340	4125986..4126357	+	372	WP_152389051.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4117421..4128012	10591

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-95)

Antitoxin

• Uncharacterized protein HI1420 (6-92)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10674.34 Da
• Isoelectric Point: 10.3110

>T141673 WP_152389045.1 NZ_CP045302:c4121648-4121358 [Azotobacter salinestris]
MPVLIQTDTYSKWFSALRDARARARINARLRRVGLGALGDCKPVGEGVSELRIDYGPGYRVYFVQRGHELVILLAGGDKS
SQDKDIKTAIKLARDL

Nucleotide

• Length: 291 bp

>T141673 NZ_CP060061:1979064-1979240 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGTGTCACCCTAGCGATGAGATTAAAGAACCATTGCGTAAAGCAATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10648.30 Da
• Isoelectric Point: 4.5836

>AT141673 WP_152389044.1 NZ_CP045302:c4121348-4121046 [Azotobacter salinestris]
MTKTTLTQWDVVDHLATEEDMALYLEACLEENDPALLAAALGDIARARGMAQLARDTGLTREGLYKALSADGNPSLATIM
KVMGALGIKLHAELVPARPA

Nucleotide

• Length: 303 bp

>AT141673 NZ_CP060061:1979286-1979702 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA