Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/couple_hipB(antitoxin)
Location 2314322..2315015 Replicon chromosome
Accession NZ_CP045302
Organism Azotobacter salinestris strain KACC 13899

Toxin (Protein)


Gene name HipA1 Uniprot ID -
Locus tag GCU53_RS25705 Protein ID WP_208845475.1
Coordinates 2314557..2315015 (+) Length 153 a.a.

Antitoxin (Protein)


Gene name HipB1 Uniprot ID -
Locus tag GCU53_RS10795 Protein ID WP_152387617.1
Coordinates 2314322..2314567 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GCU53_RS10770 2309483..2309746 + 264 WP_152387613.1 hypothetical protein -
GCU53_RS10775 2309944..2310177 + 234 WP_152387614.1 hypothetical protein -
GCU53_RS10780 2310373..2310978 - 606 WP_152387615.1 hypothetical protein -
GCU53_RS10785 2311664..2313283 + 1620 WP_152387616.1 malate:quinone oxidoreductase -
GCU53_RS10790 2313375..2314142 + 768 WP_167520062.1 haloacid dehalogenase type II -
GCU53_RS10795 2314322..2314567 + 246 WP_152387617.1 helix-turn-helix transcriptional regulator Antitoxin
GCU53_RS25705 2314557..2315015 + 459 WP_208845475.1 HipA N-terminal domain-containing protein Toxin
GCU53_RS25710 2315028..2315828 + 801 WP_208845476.1 HipA domain-containing protein -
GCU53_RS10805 2315839..2316594 - 756 WP_152387618.1 metallophosphoesterase -
GCU53_RS10810 2316676..2317500 - 825 WP_152387619.1 HNH endonuclease -
GCU53_RS25715 2317514..2319691 - 2178 WP_208845477.1 DUF3427 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2310373..2321399 11026


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-102)

Antitoxin

Transcription regulator, HipB-like

(9-65)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 16813.26 Da        Isoelectric Point: 4.9148

>T141672 WP_208845475.1 NZ_CP045302:2314557-2315015 [Azotobacter salinestris]
MAHRLEVWLFATRIGTLALVEGRLTFQYDSDWLKQPNAVALSCSLPLQAEPFDERRTRPFFAGLLPEGHLRRLIAQQLQV
SRQNDFALLDHIGGECAGAVTFLAPEQALPQPADSSEVEWLGNTQIIALLDELPRRPMLAGQDGLRLSLAGA

Download         Length: 459 bp

>T141672 NZ_CP060061:1043908-1044243 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8441.74 Da        Isoelectric Point: 8.9406

>AT141672 WP_152387617.1 NZ_CP045302:2314322-2314567 [Azotobacter salinestris]
MTTIHTTQQLGKALRAARKQLGLTQSQLALAAGVGLRFIVDLEAGKPTLRLENSLRVIEALGGEISLNGLPAPTVGDDHG
A

Download         Length: 246 bp

>AT141672 NZ_CP060061:1043660-1043908 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References