Detailed information of TA system TA141669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 4480336..4480909
• Replicon: chromosome
• Accession: NZ_CP045300
• Organism: Kosakonia arachidis strain KACC 18508

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GCU50_RS22215
• Protein ID: WP_090124640.1
• Coordinates: 4480622..4480909 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GCU50_RS22210
• Protein ID: WP_090124637.1
• Coordinates: 4480336..4480635 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU50_RS22200	4475380..4476957	+	1578	WP_090124631.1	glutamine-hydrolyzing GMP synthase	-
GCU50_RS22205	4477751..4479721	+	1971	WP_090124634.1	FIST C-terminal domain-containing protein	-
GCU50_RS22210	4480336..4480635	+	300	WP_090124637.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GCU50_RS22215	4480622..4480909	+	288	WP_090124640.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GCU50_RS22220	4480946..4481176	-	231	WP_090124643.1	helix-turn-helix domain-containing protein	-
GCU50_RS22225	4481285..4481533	+	249	WP_090124647.1	helix-turn-helix domain-containing protein	-
GCU50_RS22230	4481786..4482211	-	426	WP_090124650.1	hypothetical protein	-
GCU50_RS22235	4482208..4482750	-	543	Protein_4211	type IV secretion protein Rhs	-
GCU50_RS22240	4483243..4483503	+	261	WP_090124653.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GCU50_RS22245	4483527..4483844	+	318	WP_090124656.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4466555..4483844	17289

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-55)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10919.29 Da
• Isoelectric Point: 4.6533

>T141669 WP_090124640.1 NZ_CP045300:4480622-4480909 [Kosakonia arachidis]
VPEVEWSQSARADLLAIVDYISDDNPDAAQWLKDDIEARVAKLPEHQKLYRGGRIEGTREMVVRTNYIVVYTEDSFAVRI
LRVLHAAQQWPPAQE

Nucleotide

• Length: 288 bp

>T141669 NZ_CP060061:710733-711029 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10861.42 Da
• Isoelectric Point: 8.7733

>AT141669 WP_090124637.1 NZ_CP045300:4480336-4480635 [Kosakonia arachidis]
MAAQTSMLHIRVDDKLKADATEKLANVGLSVSDAVRIFLTRVTKEGGLPAGLTTDPEAHDKWFRAKVLEALTDSQPGVPH
QQVMDEAQALIDRKRRARG

Nucleotide

• Length: 300 bp

>AT141669 NZ_CP060061:711031-711426 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA