Detailed information of TA system TA141667

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3938812..3939475
• Replicon: chromosome
• Accession: NZ_CP045300
• Organism: Kosakonia arachidis strain KACC 18508

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: GCU50_RS19520
• Protein ID: WP_090121197.1
• Coordinates: 3939059..3939475 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: GCU50_RS19515
• Protein ID: WP_090121199.1
• Coordinates: 3938812..3939078 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU50_RS19495	3934261..3935694	-	1434	WP_090121204.1	6-phospho-beta-glucosidase	-
GCU50_RS19500	3935811..3936539	-	729	WP_090121203.1	MurR/RpiR family transcriptional regulator	-
GCU50_RS19505	3936885..3937544	+	660	WP_090121202.1	hemolysin III family protein	-
GCU50_RS19510	3937584..3938564	-	981	WP_090121200.1	tRNA-modifying protein YgfZ	-
GCU50_RS19515	3938812..3939078	+	267	WP_090121199.1	FAD assembly factor SdhE	Antitoxin
GCU50_RS19520	3939059..3939475	+	417	WP_090121197.1	protein YgfX	Toxin
GCU50_RS19525	3939495..3940016	-	522	WP_090121196.1	flavodoxin FldB	-
GCU50_RS19530	3940117..3941013	+	897	WP_090121194.1	site-specific tyrosine recombinase XerD	-
GCU50_RS19535	3941038..3941751	+	714	WP_090121193.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
GCU50_RS19540	3941757..3943490	+	1734	WP_090121191.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 16269.18 Da
• Isoelectric Point: 10.2053

>T141667 WP_090121197.1 NZ_CP045300:3939059-3939475 [Kosakonia arachidis]
VVLWQSELRVSWRAQWISLLLHGLGAALILLMPWPLSYTPLWLLLLSLVVFDCVRSQRRINACHGELKLLMDSRLRWQGV
EWDIIGAPWMLRSGMMLRLRREEDGRHQHLWLAADSMDAQEWRDLRRVISQKPAQGLH

Nucleotide

• Length: 417 bp

>T141667 NZ_CP060061:c603520-603056 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10483.95 Da
• Isoelectric Point: 5.6062

>AT141667 WP_090121199.1 NZ_CP045300:3938812-3939078 [Kosakonia arachidis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDADKQLFVRLLESDDPDLFNWLMNHGKPQDAELQRMVQLIQTRN
RDRGPVAI

Nucleotide

• Length: 267 bp

>AT141667 NZ_CP060061:c603855-603520 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA