Detailed information of TA system TA141666

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 2546682..2547395
• Replicon: chromosome
• Accession: NZ_CP045300
• Organism: Kosakonia arachidis strain KACC 18508

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: GCU50_RS12645
• Protein ID: WP_090122177.1
• Coordinates: 2546682..2547020 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GCU50_RS12650
• Protein ID: WP_090122175.1
• Coordinates: 2547045..2547395 (-)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU50_RS12615	2543019..2543567	-	549	WP_090122181.1	hypothetical protein	-
GCU50_RS12625	2544217..2545014	-	798	WP_090122180.1	helix-turn-helix domain-containing protein	-
GCU50_RS12630	2545130..2545405	-	276	WP_086530362.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GCU50_RS12635	2545409..2545657	-	249	WP_090122178.1	ribbon-helix-helix domain-containing protein	-
GCU50_RS12640	2545734..2546567	-	834	WP_090122755.1	DUF4942 domain-containing protein	-
GCU50_RS12645	2546682..2547020	-	339	WP_090122177.1	TA system toxin CbtA family protein	Toxin
GCU50_RS12650	2547045..2547395	-	351	WP_090122175.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GCU50_RS12655	2547416..2547637	-	222	WP_090122174.1	DUF987 domain-containing protein	-
GCU50_RS12660	2547651..2548133	-	483	WP_090122173.1	DNA repair protein RadC	-
GCU50_RS12665	2548169..2548990	-	822	WP_090122171.1	DUF945 domain-containing protein	-
GCU50_RS12670	2549101..2549313	-	213	WP_090122170.1	hypothetical protein	-
GCU50_RS12675	2549394..2549753	-	360	WP_090122168.1	hypothetical protein	-
GCU50_RS12680	2549807..2550271	-	465	WP_090122167.1	hypothetical protein	-
GCU50_RS12685	2550325..2551002	-	678	WP_090122165.1	WYL domain-containing protein	-
GCU50_RS12690	2551063..2551791	-	729	WP_090122164.1	DUF3944 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2528871..2564033	35162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (3-108)

Antitoxin

• Antitoxin CbeA (15-115)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12847.79 Da
• Isoelectric Point: 8.0567

>T141666 WP_090122177.1 NZ_CP045300:c2547020-2546682 [Kosakonia arachidis]
MKTPPVNQRVAKPCPPPVLVWQTLLTRLLEQHYGLTLNDTPFSDESVIQEHIDAGITLADAVNFLVEKYELVRIDRKGFS
WQEQSPYLRAVDILRARQATGLLRQSHNLLTR

Nucleotide

• Length: 339 bp

>T141666 NZ_CP060061:181280-181387 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 13370.09 Da
• Isoelectric Point: 6.2053

>AT141666 WP_090122175.1 NZ_CP045300:c2547395-2547045 [Kosakonia arachidis]
MQNKRKIAVQDIAEPWWGLRSDFSPCFSARLAQEDNQLHYLADRASFNGQFSDADLRHLDLAFPVLMKQLELMLTSGELN
PRHQHCVTLYHNELTCEADTHGSCGYVYLAIYPTQR

Nucleotide

• Length: 351 bp

>AT141666 NZ_CP060061:c181231-181168 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT