Detailed information of TA system TA141665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 2545130..2545657
• Replicon: chromosome
• Accession: NZ_CP045300
• Organism: Kosakonia arachidis strain KACC 18508

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GCU50_RS12630
• Protein ID: WP_086530362.1
• Coordinates: 2545130..2545405 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GCU50_RS12635
• Protein ID: WP_090122178.1
• Coordinates: 2545409..2545657 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU50_RS12610	2540557..2541549	-	993	WP_090122757.1	hypothetical protein	-
GCU50_RS12615	2543019..2543567	-	549	WP_090122181.1	hypothetical protein	-
GCU50_RS12625	2544217..2545014	-	798	WP_090122180.1	helix-turn-helix domain-containing protein	-
GCU50_RS12630	2545130..2545405	-	276	WP_086530362.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GCU50_RS12635	2545409..2545657	-	249	WP_090122178.1	ribbon-helix-helix domain-containing protein	Antitoxin
GCU50_RS12640	2545734..2546567	-	834	WP_090122755.1	DUF4942 domain-containing protein	-
GCU50_RS12645	2546682..2547020	-	339	WP_090122177.1	TA system toxin CbtA family protein	-
GCU50_RS12650	2547045..2547395	-	351	WP_090122175.1	type IV toxin-antitoxin system YeeU family antitoxin	-
GCU50_RS12655	2547416..2547637	-	222	WP_090122174.1	DUF987 domain-containing protein	-
GCU50_RS12660	2547651..2548133	-	483	WP_090122173.1	DNA repair protein RadC	-
GCU50_RS12665	2548169..2548990	-	822	WP_090122171.1	DUF945 domain-containing protein	-
GCU50_RS12670	2549101..2549313	-	213	WP_090122170.1	hypothetical protein	-
GCU50_RS12675	2549394..2549753	-	360	WP_090122168.1	hypothetical protein	-
GCU50_RS12680	2549807..2550271	-	465	WP_090122167.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2528871..2564033	35162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-42)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 11001.33 Da
• Isoelectric Point: 4.8888

>T141665 WP_086530362.1 NZ_CP045300:c2545405-2545130 [Kosakonia arachidis]
MEVYWTLKAQDDLERIYRFALQYSRQHADDVLDRLITGCAGLTAHPAIGVQQTRYEPREVRKVLFDDYEVHYELRDNDIY
IVDLWHTKEER

Nucleotide

• Length: 276 bp

>T141665 NZ_CP060058:c36590-36204 [Escherichia coli]
ATGAAATTCGTTAGTTCACAGGAAGTGATTGAGTTTCATGACCGCTTGATTTCGCGTGATGGTGGCGTGGCGGGTATGCC
AGAACCCGGTCGCGCTGACGCGATCATTCATCGTGTTTTGAACATGTATCATTATGAAGGTGTGACAGATATTATGGATC
TGGCTGCCGTGTATTTAGTTGCTATTGCTCGTGGTCACATTTTCAACGATGCAAATAAGCGAACAGCCTTGTTTGTTGCA
CAGGTTTTTTTAAAACGGAATGGTGTACATATTATTAGTTCACGTATTTCGTTTGATGAAATGCAAATTATCGCTTTGAA
TGCTGCAACAGGAGAATATAACTGGAAGATGGTTTCTGACCATCTTAAAGCCATTATTCTAAATTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9036.37 Da
• Isoelectric Point: 7.2042

>AT141665 WP_090122178.1 NZ_CP045300:c2545657-2545409 [Kosakonia arachidis]
MGSLTKQTISAQIPVELAAAVENLAIELDRSKSWIIKEALTSMLAERERRHQSIQAGLADVDAGRVMSHSDMVDFANRLK
KS

Nucleotide

• Length: 249 bp

>AT141665 NZ_CP060058:c36811-36587 [Escherichia coli]
ATGTTTACATGTAACTATAGCCATGCTCGCGATAACCTGGCCTCGTTGCTCGAGCTGGCGGTTGATGAAAATCAGCCAGT
TGAGATTCAGCGCCGTAAAGGTGGCGCAGCTGTACTGGTTGATAAAGACGCTTTTGAACGATACCGTAAAGCGGCTCTTG
ATGCTGAGTTTGCTGAGATCATGCAAAGTCATGGGACGGCCTATACGGAGCTTTCTAAGCGATGA