Detailed information of TA system TA141664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1863122..1863738
• Replicon: chromosome
• Accession: NZ_CP045300
• Organism: Kosakonia arachidis strain KACC 18508

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A1C4FBY4
• Locus tag: GCU50_RS09320
• Protein ID: WP_007373583.1
• Coordinates: 1863520..1863738 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: GCU50_RS09315
• Protein ID: WP_090118428.1
• Coordinates: 1863122..1863496 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU50_RS09305	1858246..1859439	+	1194	WP_090118430.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
GCU50_RS09310	1859462..1862611	+	3150	WP_090118429.1	multidrug efflux RND transporter permease subunit AcrB	-
GCU50_RS09315	1863122..1863496	+	375	WP_090118428.1	Hha toxicity modulator TomB	Antitoxin
GCU50_RS09320	1863520..1863738	+	219	WP_007373583.1	hemolysin expression modulator Hha	Toxin
GCU50_RS09325	1863804..1864341	+	538	Protein_1745	maltose O-acetyltransferase	-
GCU50_RS09330	1864442..1864921	+	480	WP_090118427.1	YlaC family protein	-
GCU50_RS09335	1864887..1866332	-	1446	WP_090118426.1	PLP-dependent aminotransferase family protein	-
GCU50_RS09340	1866429..1867130	+	702	WP_090118425.1	GNAT family N-acetyltransferase	-
GCU50_RS09345	1867127..1867267	-	141	WP_090118424.1	type B 50S ribosomal protein L36	-
GCU50_RS09350	1867271..1867531	-	261	WP_090118423.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8637.05 Da
• Isoelectric Point: 7.9893

>T141664 WP_007373583.1 NZ_CP045300:1863520-1863738 [Kosakonia arachidis]
MSEKPLTKVDYLMRLRRCQTIDTLERVIEKNKYELPDEELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 219 bp

>T141664 NZ_CP060058:28178-28459 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACCGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGAATTGGTTATTCAGCCAGCAGCCTGGCTGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14530.35 Da
• Isoelectric Point: 5.1438

>AT141664 WP_090118428.1 NZ_CP045300:1863122-1863496 [Kosakonia arachidis]
MDEYSPKRHDIAQLKFLCETLYHDCLVNLEESHHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTHDLQKWQKTGNKLFRCFVNVSKANPVSQSC

Nucleotide

• Length: 375 bp

>AT141664 NZ_CP060058:27912-28181 [Escherichia coli]
ATGACACAGGTTAACATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGCGGCGATGTGCTTTCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A1C4FBY4