Detailed information of TA system TA141653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 22056..22591
• Replicon: plasmid unnamed1
• Accession: NZ_CP045299
• Organism: Kosakonia arachidis strain KACC 18508

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GCU50_RS00080
• Protein ID: WP_090127026.1
• Coordinates: 22056..22343 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GCU50_RS00085
• Protein ID: WP_090127024.1
• Coordinates: 22340..22591 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU50_RS00045	17760..18017	+	258	WP_090127048.1	hypothetical protein	-
GCU50_RS00050	18252..18581	+	330	WP_090127046.1	thioredoxin family protein	-
GCU50_RS00055	18654..19442	+	789	WP_090127055.1	transporter substrate-binding domain-containing protein	-
GCU50_RS00060	19454..20119	+	666	WP_090127045.1	amino acid ABC transporter permease	-
GCU50_RS00070	20796..21578	-	783	WP_090127043.1	conjugal transfer protein TraX	-
GCU50_RS00075	21604..21981	+	378	Protein_12	DDE-type integrase/transposase/recombinase	-
GCU50_RS00080	22056..22343	-	288	WP_090127026.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GCU50_RS00085	22340..22591	-	252	WP_090127024.1	antitoxin	Antitoxin
GCU50_RS00090	22983..24323	+	1341	WP_090127022.1	hypothetical protein	-
GCU50_RS00095	24414..24722	-	309	WP_090127020.1	hypothetical protein	-
GCU50_RS00100	24725..25360	-	636	WP_090127052.1	AAA family ATPase	-
GCU50_RS00105	26101..26862	-	762	WP_090127018.1	replication initiation protein RepE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..79613	79613

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11102.04 Da
• Isoelectric Point: 10.4701

>T141653 WP_090127026.1 NZ_CP045299:c22343-22056 [Kosakonia arachidis]
MSYAVKFREDALKEWQKLDKTIQQQFAKKLKKCSENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDNELIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T141653 NZ_CP060057:c2546048-2545719 [Escherichia coli]
ATGAACTACATTATCGAATACTACAGTGACGAGGTTGAAGCTGAGATCCTTTCTCTTCCTGAGACCTTACAAGCCCGGTA
CATTCGGTACACGGAGAAAATGCGTATTTACGGAGCTAATCTTGGTTCCCCGCATACAGAGGCATTTGGCGACGGTTTGT
TTGAAATCCGCCTTAAGGGCTCGGAAGGCATAGGGCGTGTTTTTTACTGCACGCTAAAAGGAAAGCGAATCATTATGTTG
CATAGCTTTGTAAAGAAAACGCAAAAAACTCCACCTGCCGAGCTTAGAAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9160.45 Da
• Isoelectric Point: 4.0390

>AT141653 WP_090127024.1 NZ_CP045299:c22591-22340 [Kosakonia arachidis]
MPSIILSETSASVSELKKNPMATVSAGNGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVTERSNQQLLDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT141653 NZ_CP060057:c2545729-2545421 [Escherichia coli]
ATGACTGGTAAACGTACCCCACCTACCATGACACACGATGATATGGTAGAAAAAATGTTGTCTAACCCCGCAGTAAAAGC
AGAATACGACGCCATCGCCGATGAATTCGCACTACTTGATGAAATGCTGGCAGCACGAAAAGAAGCTGGCTTAACCCAGG
CTGAAGTTGCTGAACGTATGGGAACAAAAGCGACTGCGATAACCAGAATGGAAAGCAATCTTGCATCAGGTACAAGCGGC
CCATCATTTGCCACACTGAAAAAATTCGCCCGTGCTACAGGGAAAAAACTCCAGATCCGCTTCGTTTAA