Detailed information of TA system TA141634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 30785..31583
• Replicon: plasmid pLAU-OXA1
• Accession: NZ_CP045278
• Organism: Escherichia coli strain LAU-OXA

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: GBF15_RS23665
• Protein ID: WP_023908317.1
• Coordinates: 31062..31583 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A0V9R6Q7
• Locus tag: GBF15_RS23660
• Protein ID: WP_001351987.1
• Coordinates: 30785..31054 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBF15_RS23635	25906..27246	-	1341	WP_000137333.1	AAA family ATPase	-
GBF15_RS23640	27290..28030	-	741	WP_001717320.1	hypothetical protein	-
GBF15_RS23645	28320..29378	+	1059	WP_113519370.1	hypothetical protein	-
GBF15_RS23650	29439..29792	-	354	WP_160378290.1	hypothetical protein	-
GBF15_RS23655	29798..30466	-	669	WP_000161228.1	AAA family ATPase	-
GBF15_RS23660	30785..31054	+	270	WP_001351987.1	DUF1778 domain-containing protein	Antitoxin
GBF15_RS23665	31062..31583	+	522	WP_023908317.1	GNAT family N-acetyltransferase	Toxin
GBF15_RS23675	31751..32002	-	252	WP_001404395.1	hypothetical protein	-
GBF15_RS23680	32004..32696	-	693	WP_000856757.1	hypothetical protein	-
GBF15_RS23685	32710..33033	-	324	WP_001717323.1	hypothetical protein	-
GBF15_RS23695	33356..33964	-	609	WP_031323050.1	hypothetical protein	-
GBF15_RS23700	33964..34218	-	255	WP_000120169.1	hypothetical protein	-
GBF15_RS23705	34381..34908	-	528	WP_023908314.1	tail fiber assembly protein	-
GBF15_RS23710	34911..35825	-	915	WP_113519376.1	phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-15	-	1..111694	111694

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19497.36 Da
• Isoelectric Point: 8.9815

>T141634 WP_023908317.1 NZ_CP045278:31062-31583 [Escherichia coli]
VDNIKIEIFSGEKHYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEGRPKVLGYYTLSGSCFEKESLPSRSQQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLDFIQLVGNNERSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T141634 NZ_CP060049:c4686519-4686178 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGACGCTATGTCTGTCGCCCGTGTCTGTCTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCGTTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGAGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10082.54 Da
• Isoelectric Point: 7.0155

>AT141634 WP_001351987.1 NZ_CP045278:30785-31054 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Nucleotide

• Length: 270 bp

>AT141634 NZ_CP060049:c4686881-4686540 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTAACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATGTGGCATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACCGCGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACTCCACCCATCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0V9R6Q7