Detailed information of TA system TA141632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4243849..4244444
• Replicon: chromosome
• Accession: NZ_CP045277
• Organism: Escherichia coli strain LAU-OXA

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: GBF15_RS20800
• Protein ID: WP_000239581.1
• Coordinates: 4243849..4244199 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: GBF15_RS20805
• Protein ID: WP_001223213.1
• Coordinates: 4244193..4244444 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBF15_RS20780	4239296..4240318	-	1023	WP_001586590.1	ABC transporter permease	-
GBF15_RS20785	4240332..4241834	-	1503	Protein_4001	sugar ABC transporter ATP-binding protein	-
GBF15_RS20790	4241974..4242930	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
GBF15_RS20795	4243240..4243770	+	531	WP_000055075.1	inorganic diphosphatase	-
GBF15_RS20800	4243849..4244199	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
GBF15_RS20805	4244193..4244444	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
GBF15_RS20810	4244656..4244997	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
GBF15_RS20815	4245000..4248779	-	3780	WP_021564810.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T141632 WP_000239581.1 NZ_CP045277:c4244199-4243849 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T141632 NZ_CP060049:4047720-4047938 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATTCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT141632 WP_001223213.1 NZ_CP045277:c4244444-4244193 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT141632 NZ_CP060049:4047319-4047693 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7